Pubblicazione scientifica

A Hopeful Sea-Monster: A Very Large Homologous Recombination Event Impacting the Core Genome of the Marine Pathogen Vibrio anguillarum

FILONE TECNOLOGICO	TEMA
economia circolare e sostenibilità

RIASSUNTO

Vibrio anguillarum is the causative agent of vibriosis in many species important to aquaculture. We generated whole genome sequence (WGS) data on a diverse collection of 64 V. anguillarum strains, which we supplemented with 41 publicly available genomes to produce a combined dataset of 105 strains. These WGS data resolved six major lineages (L1-L6), and the additional use of multilocus sequence analysis (MLSA) clarified the association of L1 with serotype O1 and Salmonidae hosts (salmon/trout), and L2 with serotypes O2a/O2b/O2c and Gadidae hosts (cod). Our analysis also revealed a large-scale homologous replacement of 526-kb of core genome in an L2 strain from a con-specific donor. Although the strains affected by this recombination event are exclusively associated with Gadidae, we find no clear genetic evidence that it has played a causal role in host specialism. Whilst it is established that Vibrio species freely recombine, to our knowledge this is the first report of a contiguous recombinational replacement of this magnitude in any Vibrio genome. We also note a smaller accessory region of high single nucleotide polymorphism (SNP) density and gene content variation that contains lipopolysaccharide biosynthesis genes which may play a role in determining serotype.

DATA

Data di pubblicazione: 29/06/2020

AUTORI

COYLE NICOLA M
BARTIE KERRY L
BAYLISS SION C
BEKAERT MICHAEL
ADAMS ALEXANDRA
MCMILLAN STUART
VERNER-JEFFREYS DAVID W
DESBOIS ANDREW P
FEIL EDWARD J

ENTE DI AFFERENZA

MILNER CTR EVOLUTION DEPT BIOLOGY & BIOCHEMISTRY UNIV BATH BATH UK
INST AQUACULTURE UNIV STIRLING STIRLING UK
CEFAS WEYMOUTH LAB WEYMOUTH UK

RIVISTA

Frontiers in Microbiology, 11, 1430

IMPACT FACTOR

4,190

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