Pubblicazione scientifica

Isolation and Characterization of Acinetobacter baumannii Recovered from Campylobacter Selective Medium

FILONE TECNOLOGICO	TEMA
produzione e raccolta

RIASSUNTO

Acinetobacter baumannii, a Gram-negative opportunistic pathogen, is known to cause multidrug resistant infections. This organism has primarily been isolated from clinical environments and its environmental reservoirs remain largely unknown. In the present study, we recovered seven isolates of A. baumannii growing under conditions selective for Campylobacter spp. (microaerophilic at 42°C and in the presence of antibiotics) from dairy cattle manure storage tank or surface water impacted by livestock effluents. Antibiotic susceptibility tests revealed that all of these isolates were less susceptible to at least two different clinically relevant antibiotics, compared to the type strain A. baumannii ATCC17978. Expression of resistance-nodulation-division efflux pumps, an important mechanism of intrinsic resistance in these organisms, was analyzed, and adeB was found to be overexpressed in one and adeJ was overexpressed in three isolates. Comparison of these isolates using genomic DNA Macro-Restriction Fragment Pattern Analysis (MRFPA) revealed relatively low relatedness among themselves or with some of the clinical isolates from previous studies. This study suggests that A. baumannii isolates are capable of growing under selective conditions for Campylobacter spp. and that this organism can be present in manure and water.

DATA

Data di pubblicazione: 18/11/2016

AUTORI

FERNANDO DINESH M
KHAN IZHAR U H
PATIDAR RAKESH
LAPEN DAVID R
TALBOT GUYLAINE
TOPP EDWARD
KUMAR AYUSH

ENTE DI AFFERENZA

DEPT MICROBIOLOGY UNIV MANITOBA WINNIPEG MB CANADA
OTTAWA RSCH & DEV CTR AGRICULTURE & AGRI FOOD CANADA OTTAWA ON CANADA
SHERBROOKE RSCH & DEV CTR AGRICULTURE & AGRI FOOD CANADA SHERBROOKE QC CANADA
LONDON RSCH & DEV CTR AGRICULTURE & AGRI FOOD CANADA LONDON ON CANADA
DEPT MEDICAL MICROBIOLOGY UNIV MANITOBA WINNIPEG MB CANADA

RIVISTA

Frontiers in Microbiology, 7, 1871

IMPACT FACTOR

4,190

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