Pubblicazione scientifica

Perchlorate Exposure Reduces Primordial Germ Cell Number in Female Threespine Stickleback

FILONE TECNOLOGICO	TEMA
produzione e raccolta
trasformazione e commercializzazione

RIASSUNTO

Perchlorate is a common aquatic contaminant that has long been known to affect thyroid function in vertebrates, including humans. More recently perchlorate has been shown to affect primordial sexual differentiation in the aquatic model fishes zebrafish and threespine stickleback, but the mechanism has been unclear. Stickleback exposed to perchlorate from fertilization have increased androgen levels in the embryo and disrupted reproductive morphologies as adults, suggesting that perchlorate could disrupt the earliest stages of primordial sexual differentiation when primordial germ cells (PGCs) begin to form the gonad. Female stickleback have three to four times the number of PGCs as males during the first weeks of development. We hypothesized that perchlorate exposure affects primordial sexual differentiation by reducing the number of germ cells in the gonad during an important window of stickleback sex determination at 14–18 days post fertilization (dpf). We tested this hypothesis by quantifying the number of PGCs at 16 dpf in control and 100 mg/L perchlorate-treated male and female stickleback. Perchlorate exposure from the time of fertilization resulted in significantly reduced PGC number only in genotypic females, suggesting that the masculinizing effects of perchlorate observed in adult stickleback may result from early changes to the number of PGCs at a time critical for sex determination. To our knowledge, this is the first evidence of a connection between an endocrine disruptor and reduction in PGC number prior to the first meiosis during sex determination. These findings suggest that a mode of action of perchlorate on adult reproductive phenotypes in vertebrates, including humans, such as altered fecundity and sex reversal or intersex gonads, may stem from early changes to germ cell development.

DATA

Data di pubblicazione: 06/07/2016

AUTORI

PETERSEN ANN M
EARP NATHANIAL C
REDMOND MANDY E
POSTLETHWAIT JOHN H
VON HIPPEL FRANK A
BUCK C LOREN
CRESKO WILLIAM A

ENTE DI AFFERENZA

INST ECOLOGY & EVOLUTION DEPT BIOLOGY UNIV OREGON EUGENE OREGON 97403 USA
DEPT INTEGRATIVE BIOLOGY OREGON STATE UNIV CASCADES BEND OREGON 97703 USA
INST NEUROSCIENCE DEPT BIOLOGY UNIV OREGON EUGENE OREGON 97403 USA
DEPT BIOLOGICAL SCIENCES NORTHERN ARIZONA UNIV FLAGSTAFF ARIZONA 86001 USA
DEPT BIOLOGICAL SCIENCES & CTR BIOENGINEERING INNOV NORTHERN ARIZONA UNIV FLAGSTAFF ARIZONA 86001 USA
UNIV ROUEN FRANCE FRANCE

RIVISTA

PLoS ONE, 11(7), e0157792

IMPACT FACTOR

2,870

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