DESCRIZIONE
A method of microbial cell disruption for use in making an aquaculture feed composition is disclosed, wherein a microbial biomass having a moisture level less than 10 weight percent and comprising oil-containing microbes is disrupted, resulting in a disruption efficiency of at least 30% of the oil-containing microbes to produce a disrupted microbial biomass, and, the disrupted microbial biomass is mixed with at least one aquaculture feed component to form an aquaculture feed composition.