Pubblicazione scientifica

A Novel Chitin Binding Crayfish Molar Tooth Protein with Elasticity Properties

FILONE TECNOLOGICO	TEMA
produzione e raccolta

RIASSUNTO

The molar tooth of the crayfish Cherax quadricarinatus is part of the mandible, and is covered by a layer of apatite (calcium phosphate). This tooth sheds and is regenerated during each molting cycle together with the rest of the exoskeleton. We discovered that molar calcification occurs at the pre-molt stage, unlike calcification of the rest of the new exoskeleton. We further identified a novel molar protein from C. quadricarinatus and cloned its transcript from the molar-forming epithelium. We termed this protein Cq-M13. The temporal level of transcription of Cq-M13 in an NGS library of molar-forming epithelium at different molt stages coincides with the assembly and mineralization pattern of the molar tooth. The predicted protein was found to be related to the pro-resilin family of cuticular proteins. Functionally, in vivo silencing of the transcript caused molt cycle delay and a recombinant version of the protein was found to bind chitin and exhibited elastic properties.

DATA

Data di pubblicazione: 26/05/2015

AUTORI

TYNYAKOV JENNY
BENTOV SHMUEL
ABEHSERA SHAI
KHALAILA ISAM
MANOR RIVKA
KATZIR ABILEVICH LIHIE
WEIL SIMY
AFLALO ELIAHU D
SAGI AMIR

ENTE DI AFFERENZA

DEPT LIFE SCIENCES BEN GURION UNIV BEER SHEVA ISRAEL
NATL INST BIOTECHNOLOGY IN NEGEV BEN GURION UNIV BEER SHEVA ISRAEL
DEPT BIOTECHNOLOGY ENG BEN GURION UNIV BEER SHEVA ISRAEL
UPPSALA UNIV SWEDEN

RIVISTA

PLoS ONE, 10(5), e0127871

IMPACT FACTOR

2,870

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