Pubblicazione scientifica

Development of Cryopreservation Techniques for Gorgonian (Junceella juncea) Oocytes through Vitrification

FILONE TECNOLOGICO	TEMA
produzione e raccolta

RIASSUNTO

Gorgonian corals are slowly declining due to human interaction and environmental impacts. Cryopreservation of gorgonian corals is an ex-situ method of conservation, ensuring future reproduction. The present study assessed the vitrification properties of cryoprotectant (CPT) mixtures using the cryotop, cryoloop and open pulled straw (OPS) cryopereservation methods prior to experimentation on gorgonian (Junceella juncea) oocytes. Investigations of the equilibration and vitrification solutions’ (ES and VS) effect on oocytes throughout different incubation periods were conducted. The cryotop method was found to be the most successful in ensuring vitrification. The most favourable VS was composed of propylene glycol (PG), ethylene glycol (EG) and methanol with concentrations of 3.5M, 1.5M and 2M respectively. Experiments were performed using the cryotop method to cryopreserve Junceella juncea oocytes using VS2, the solution had the least impact on oocytes at 5°C rather than at 26°C. The success of the vitrification procedures was determined by adenosine triphosphate (ATP) levels in cooled-thaw oocytes and the highest viability obtained from the present study was 76.6 ± 6.2%. This study provides information regarding gorgonian corals’ tolerance and viability throughout vitrification to further advance the vitrification protocol on whip corals.

DATA

Data di pubblicazione: 26/05/2015

AUTORI

TSAI SUJUNE
YEN WISH
CHAVANICH SUCHANA
VIYAKARN VORANOP
LIN CHIAHSIN

ENTE DI AFFERENZA

DEPT BIOTECHNOLOGY MINGDAO UNIV PEETOW CHANG HUA TAIWAN
DEPT POST MODERN AGRICULTURE MINGDAO UNIV PEETOW CHANG HUA TAIWAN
INST BIOCHEMISTRY & ENVIRONMENTAL SCIENCE UNIV OTTAWA OTTAWA ON CANADA
REEF BIOLOGY RSCH GROUP DEPT MARINE SCIENCE FACULTY OF SCIENCE CHULALONGKORN UNIV BANGKOK THAILAND
NATL MUSEUM OF MARINE BIOLOGY & AQUARIUM CHECHENG PINGTUNG TAIWAN
INST
KANSAS STATE UNIV US

RIVISTA

PLoS ONE, 10(5), e0123409

IMPACT FACTOR

2,870

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