Pubblicazione scientifica

In vitro inhibition of Cyprinid herpesvirus-3 replication by RNAi

FILONE TECNOLOGICO	TEMA
produzione e raccolta
trasformazione e commercializzazione

RIASSUNTO

Cyprinid herpesvirus-3 (CyHV-3) is an etiological agent of a notifiable disease that causes high mortality rates affecting both the common and koi carp Cyprinus carpio L. There is no current treatment strategy to save CyHV-3 infected fish. RNA mediated interference (RNAi) is an emerging strategy used for understanding gene function and is a promising method in developing novel therapeutics and antiviral medications. For this study, the possibility of activating the RNAi pathway by the use of small interfering (si)RNAs was tested to inhibit in vitro viral replication of CyHV-3 in common carp brain (CCB) cells. The siRNAs were designed to target either thymidine kinase (TK) or DNA polymerase (DP) genes, which both code for transcripts involved in DNA replication. The inhibition of viral replication caused by the siRNAs was measured by a reporter gene, termed ORF81. Treatment with siRNA targeting either TK or DP genes reduced the release of viral particles from infected CCB cells. However, siRNA targeting DP was most effective at reducing viral release as measured by qPCR.

DATA

Data di pubblicazione: 15/09/2014

AUTORI

GOTESMAN MICHAEL
SOLIMAN HATEM
BESCH ROBERT
EL-MATBOULI MANSOUR

ENTE DI AFFERENZA

CLINICAL
FISH MEDICINE & MGMT FACULTY OF VETERINARY MEDICINE UNIV ASSIUT 71515 ASSIUT EGYPT
CLINIC & POLICLINIC FOR DERMATOLOGY & ALLERGOLOGY DEPT DERMATOLOGY LUDWIG MAXIMILIAN UNIV MUNICH GERMANY

RIVISTA

Journal of Virological Methods, 206(100), 63-66

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