Pubblicazione scientifica

Structural Elucidation of Novel Saponins in the Sea Cucumber Holothuria lessoni

FILONE TECNOLOGICO	TEMA
produzione e raccolta

RIASSUNTO

Sea cucumbers are prolific producers of a wide range of bioactive compounds. This study aimed to purify and characterize one class of compound, the saponins, from the viscera of the Australian sea cucumber Holothuria lessoni. The saponins were obtained by ethanolic extraction of the viscera and enriched by a liquid-liquid partition process and adsorption column chromatography. A high performance centrifugal partition chromatography (HPCPC) was applied to the saponin-enriched mixture to obtain saponins with high purity. The resultant purified saponins were profiled using MALDI-MS/MS and ESI-MS/MS which revealed the structure of isomeric saponins to contain multiple aglycones and/or sugar residues. We have elucidated the structure of five novel saponins, Holothurins D/E and Holothurinosides X/Y/Z, along with seven reported triterpene glycosides, including sulfated and non-sulfated saponins containing a range of aglycones and sugar moieties, from the viscera of H. lessoni. The abundance of novel compounds from this species holds promise for biotechnological applications.

DATA

Data di pubblicazione: 08/08/2014

AUTORI

BAHRAMI YADOLLAH
ZHANG WEI
CHATAWAY TIM
FRANCO CHRIS

ENTE DI AFFERENZA

DEPT MEDICAL BIOTECHNOLOGY SCHOOL OF MEDICINE FLINDERS UNIV ADELAIDE SA 5042 AUSTRALIA E MAILS YADOLLAH BAHRAMI FLINDERS EDU AU YB WEI ZHANG FLINDERS EDU AU WZ
CTR MARINE BIOPRODUCTS DEV FLINDERS UNIV ADELAIDE SA 5042 AUSTRALIA
AUSTRALIAN SEAFOOD COOPERATIVE RSCH CTR MARK OLIPHANT BLDG
MEDICAL BIOLOGY RSCH CTR KERMANSHAH UNIV MEDICAL SCIENCES KERMANSHAH 6714415185 IRAN
FLINDERS PROTEOMICS FACILITY SCHOOL OF MEDICINE FLINDERS UNIV ADELAIDE SA 5042 AUSTRALIA E MAIL TIM CHATAWAY FLINDERS EDU AU

RIVISTA

Marine Drugs, 12(8), 4439-4473

IMPACT FACTOR

3,810

indietro