Pubblicazione scientifica

Strategies and hurdles using DNA vaccines to fish

FILONE TECNOLOGICO	TEMA
economia circolare e sostenibilità
produzione e raccolta
trasformazione e commercializzazione

RIASSUNTO

DNA vaccinations against fish viral diseases as IHNV at commercial level in Canada against VHSV at experimental level are both success stories. DNA vaccination strategies against many other viral diseases have, however, not yet yielded sufficient results in terms of protection. There is an obvious need to combat many other viral diseases within aquaculture where inactivated vaccines fail. There are many explanations to why DNA vaccine strategies against other viral diseases fail to induce protective immune responses in fish. These obstacles include: 1) too low immunogenicity of the transgene, 2) too low expression of the transgene that is supposed to induce protection, 3) suboptimal immune responses, and 4) too high degradation rate of the delivered plasmid DNA. There are also uncertainties with regard distribution and degradation of DNA vaccines that may have implications for safety and regulatory requirements that need to be clarified. By combining plasmid DNA with different kind of adjuvants one can increase the immunogenicity of the transgene antigen – and perhaps increase the vaccine efficacy. By using molecular adjuvants with or without in combination with targeting assemblies one may expect different responses compared with naked DNA. This includes targeting of DNA vaccines to antigen presenting cells as a central factor in improving their potencies and efficacies by means of encapsulating the DNA vaccine in certain carriers systems that may increase transgene and MHC expression. This review will focus on DNA vaccine delivery, by the use of biodegradable PLGA particles as vehicles for plasmid DNA mainly in fish.

DATA

Data di pubblicazione: 01/01/2014

AUTORI

HØLVOLD LINN B
MYHR ANNE I
DALMO ROY A

ENTE DI AFFERENZA

Genøk – Centre for Biosafety The Science Park Postbox 6418 9294 Tromsø Norway
UIT ARCTIC UNIV NORWAY FACULTY OF BIOSCIENCES FISHERIES & ECONOMICS NORWEGIAN COLLEGE OF FISHERY SCIENCE 9037 TROMS NORWAY

RIVISTA

Veterinary Research, 45(1), 21

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