Pubblicazione scientifica

Association of Vitamin E with Rapid Thawing on Goat Semen

FILONE TECNOLOGICO	TEMA
produzione e raccolta

RIASSUNTO

The aim of this study was to evaluate the effects of vitamin E associated with rapid thawing on cryopreserved goat semen. Two bucks were used and eight ejaculates per animal were collected using artificial vagina. Semen was diluted with the following treatments: BIOXCELL (control), BIOXCELL + Equex (sodium lauryl sulphate) and BIOXCELL + vitamin E 100 μM. Semen was packaged into 0.25 mL straws and cooled at 5°C for 1 hour. Freezing was performed in liquid nitrogen vapor (−155°C) during 15 minutes. Then, the straws were immersed in liquid nitrogen (−196°C). Straws were thawed at 38°C/60 seconds or at 60°C/7 seconds with immediate sperm analysis. Hypoosmotic swelling test was performed adding a 20 μL aliquot of thawed semen to 1 mL of hypoosmotic solution (100 mOsm·Kg−1) followed by incubation during 60 minutes in water bath (38°C). Vitamin E did not affect any studied parameters (P > 0.05). Nevertheless, defrosting rate of 60°C/7 seconds improved sperm membrane functional integrity (P < 0.05). Current knowledge about goat semen cryopreservation is not sufficient to ensure high post-thawing recovery rates; thus, this study brings important data about using antioxidants and different thawing rates on cryopreservation process.

DATA

Data di pubblicazione: 01/01/2014

AUTORI

PENITENTE-FILHO JURANDY MAURO
OLIVEIRA FABRICIO ALBANI
JIMENEZ CAROLINA RODRIGUEZ
CARRASCAL ERLY
DIAS JULIO CESAR OLIVEIRA
OLIVEIRA GISELE DIAS
SILVEIRA RENATA GOMES
SILVEIRA CAMILA OLIVEIRA
TORRES CIRO ALEXANDRE ALVES

ENTE DI AFFERENZA

DEPT ANIMAL SCIENCE UNIV FEDERAL DE VICOSA 36570 900 VICOSA MG BRAZIL
LAB OF PHYSIOLOGY & ANIMAL REPRODUCTION DEPT ANIMAL SCIENCE UNIV FEDERAL DE VICOSA PH
DEPT VETERINARY FEDERAL UNIV VICOSA PH
FEDERAL UNIV ESPIRITO SANTO UNIV CAMPUS 29500 000 ALEGRE ES BRAZIL

RIVISTA

The Scientific World Journal, 2014, 964172

IMPACT FACTOR

1,460

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