Pubblicazione scientifica

Highly sensitive protein detection by biospecific AFM‐based fishing with pulsed electrical stimulation

FILONE TECNOLOGICO	TEMA
economia circolare e sostenibilità
produzione e cattura

RIASSUNTO

We report here the highly sensitive detection of protein in solution at concentrations from 10–15 to 10–18m using the combination of atomic force microscopy ( AFM) and mass spectrometry. Biospecific detection of biotinylated bovine serum albumin was carried out by fishing out the protein onto the surface of AFM chips with immobilized avidin, which determined the specificity of the analysis. Electrical stimulation was applied to enhance the fishing efficiency. A high sensitivity of detection was achieved by application of nanosecond electric pulses to highly oriented pyrolytic graphite placed under the AFM chip. A peristaltic pump‐based flow system, which is widely used in routine bioanalytical assays, was employed throughout the analysis. These results hold promise for the development of highly sensitive protein detection methods using nanosensor devices.

DATA

Data di pubblicazione: 10/07/2017

AUTORI

PLESHAKOVA TATYANA O
MALSAGOVA KRISTINA A
KAYSHEVA ANNA L
KOPYLOV ARTHUR T
TATUR VADIM YU
ZIBOROV VADIM S
KANASHENKO SERGEY L
GALIULLIN RAFAEL A
IVANOV YURI D

ENTE DI AFFERENZA

INST BIOMEDICAL CHEMISTRY MOSCOW RUSSIA
FNDN PERSPECTIVE TECH & NOVATIONS MOSCOW RUSSIA
JOINT INST HIGH TEMPERATURES RAS MOSCOW RUSSIA

RIVISTA

FEBS Open Bio, 7(8), 1186-1195

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