Pubblicazione scientifica

Validation of a portable, waterproof blood pH analyser for elasmobranchs

FILONE TECNOLOGICO	TEMA
economia circolare e sostenibilità
produzione e cattura
trasformazione e commercializzazione

RIASSUNTO

Abstract

Quantifying changes in blood chemistry in elasmobranchs can provide insights into the physiological insults caused by anthropogenic stress, and can ultimately inform conservation and management strategies. Current methods for analysing elasmobranch blood chemistry in the field are often costly and logistically challenging. We compared blood pH values measured using a portable, waterproof pH meter (Hanna Instruments HI 99161) with blood pH values measured by an i-STAT system (CG4+ cartridges), which was previously validated for teleost and elasmobranch fishes, to gauge the accuracy of the pH meter in determining whole blood pH for the Cuban dogfish (Squalus cubensis) and lemon shark (Negaprion brevirostris). There was a significant linear relationship between values derived via the pH meter and the i-STAT for both species across a wide range of pH values and temperatures (Cuban dogfish: 6.8–7.1 pH 24–30°C; lemon sharks: 7.0–7.45 pH 25–31°C). The relative error in the pH meter's measurements was ~±2.7%. Using this device with appropriate correction factors and consideration of calibration temperatures can result in both a rapid and accurate assessment of whole blood pH, at least for the two elasmobranch species examined here. Additional species should be examined in the future across a wide range of temperatures to determine whether correction factors are universal.

DATA

Data di pubblicazione: 27/02/2017

AUTORI

TALWAR BRENDAN
BOUYOUCOS IAN A
SHIPLEY OLIVER
RUMMER JODIE L
MANDELMAN JOHN W
BROOKS EDWARD J
GRUBBS R DEAN

ENTE DI AFFERENZA

COASTAL & MARINE LAB
DEPT NATURAL RESOURCES & ENVIRONMENTAL SCIENCES UNIV ILLINOIS AT URBANA CHAMPAIGN URBANA IL 61801 USA
ARC CTR EXCELLENCE FOR CORAL REEF STUDIES JAMES COOK UNIV TOWNSVILLE QUEENSLAND 4811 AUSTRALIA
SCHOOL OF MARINE & ATMOSPHERIC SCIENCES STONY BROOK UNIV STONY BROOK NY 11790 USA
JOHN H PRESCOTT MARINE LAB
SHARK RSCH & CONSERVATION PROGRAM CAPE ELEUTHERA INST ROCK SOUND BAHAMAS

RIVISTA

Conservation Physiology, 5(1), cox012

IMPACT FACTOR

3,150

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