Pubblicazione scientifica

Evaluation of Genotoxic Pressure along the Sava River

FILONE TECNOLOGICO	TEMA
produzione e cattura
trasformazione e commercializzazione

RIASSUNTO

In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg—modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg—modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).

DATA

Data di pubblicazione: 15/09/2016

AUTORI

KOLAREVIC STOIMIR
ABORGIBA MUSTAFA
KRACUN-KOLAREVIC MARGARETA
KOSTIC JOVANA
SIMONOVIC PREDRAG
SIMIC VLADICA
MILOSKOVIC ALEKSANDRA
REISCHER GEORG
FARNLEITNER ANDREAS
GACIC ZORAN
MILACIC RADMILA
ZULIANI TEA
VIDMAR JANJA
PERGAL MARIJA
PIRIA MARINA
PAUNOVIC MOMIR
VUKOVIC-GACIC BRANKA

ENTE DI AFFERENZA

University of Belgrade Institute for Biological Research ¨Siniša Stanković¨ Belgrade Serbia
Department of Environmental Sciences Jožef Stefan Institute Ljubljana Slovenia
UNIV BELGRADE FACULTY OF BIOLOGY CHAIR OF MICROBIOLOGY CTR GENOTOXICOLOGY & ECOGENOTOXICOLOGY BELGRADE SERBIA
UNIV BELGRADE INST MULTIDISCIPLINARY RSCH BELGRADE SERBIA
UNIV BELGRADE FACULTY OF BIOLOGY INST ZOOLOGY BELGRADE SERBIA
UNIV KRAGUJEVAC FACULTY OF SCIENCE KRAGUJEVAC SERBIA
TU WIEN INST CHEM ENG RSCH GROUP ENVIRONMENTAL MICROBIOLOGY & MOLECULAR ECOLOGY INTERUNIVERSITY COOPERATION CTR WATER & HEALTH VIENNA AUSTRIA
UNIV BELGRADE INST CHEMISTRY TECH & METALLURGY BELGRADE SERBIA
UNIV ZAGREB FACULTY OF AGRICULTURE ZAGREB CROATIA
JINLING INST TECH CHINA

RIVISTA

PLoS ONE, 11(9), e0162450

IMPACT FACTOR

2,870

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