Pubblicazione scientifica

Between Amyloids and Aggregation Lies a Connection with Strength and Adhesion

FILONE TECNOLOGICO	TEMA
produzione e cattura

RIASSUNTO

We tell of a journey that led to discovery of amyloids formed by yeast cell adhesins and their importance in biofilms and host immunity. We begin with the identification of the adhesin functional amyloid-forming sequences that mediate fiber formation in vitro. Atomic force microscopy and confocal microscopy show 2-dimensional amyloid “nanodomains” on the surface of cells that are activated for adhesion. These nanodomains are arrays of adhesin molecules that bind multivalent ligands with high avidity. Nanodomains form when adhesin molecules are stretched in the AFM or under laminar flow. Treatment with antiamyloid perturbants or mutation of the amyloid sequence prevents adhesion nanodomain formation and activation. We are now discovering biological consequences. Adhesin nanodomains promote formation and maintenance of biofilms, which are microbial communities. Also, in abscesses within candidiasis patients, we find adhesin amyloids on the surface of the fungi. In both human infection and a Caenorhabditis elegans infection model, the presence of fungal surface amyloids elicits anti-inflammatory responses. Thus, this is a story of how fungal adhesins respond to extension forces through formation of cell surface amyloid nanodomains, with key consequences for biofilm formation and host responses.

DATA

Data di pubblicazione: 01/01/2014

AUTORI

LIPKE PETER N
RAMSOOK CALEEN
GARCIA-SHERMAN MELISSA C
JACKSON DESMOND N
CHAN CHO X J
BOIS MICHAEL
KLOTZ STEPHEN A

ENTE DI AFFERENZA

BIOLOGY DEPT BROOKLYN COLLEGE CITY UNIV NEW YORK
GRADUATE CTR CITY UNIV NEW YORK NEW YORK NY 10016 USA
SECTION OF INFECTIOUS DISEASES UNIV ARIZONA TUCSON AZ 85724 USA

RIVISTA

New journal of science, 2014, 815102

indietro