Pubblicazione scientifica

Systems glycomics of adult zebrafish identifies organ-specific sialylation and glycosylation patterns

FILONE TECNOLOGICO	TEMA
produzione e cattura

RIASSUNTO

The emergence of zebrafish Danio rerio as a versatile model organism provides the unique opportunity to monitor the functions of glycosylation throughout vertebrate embryogenesis, providing insights into human diseases caused by glycosylation defects. Using a combination of chemical modifications, enzymatic digestion and mass spectrometry analyses, we establish here the precise glycomic profiles of eight individual zebrafish organs and demonstrate that the protein glycosylation and glycosphingolipid expression patterns exhibits exquisite specificity. Concomitant expression screening of a wide array of enzymes involved in the synthesis and transfer of sialic acids shows that the presence of organ-specific sialylation motifs correlates with the localized activity of the corresponding glycan biosynthesis pathways. These findings provide a basis for the rational design of zebrafish lines expressing desired glycosylation profiles.

DATA

Data di pubblicazione: 07/11/2018

AUTORI

YAMAKAWA NAO
VANBESELAERE JORICK
CHANG LAN-YI
YU SHIN-YI
DUCROCQ LUCIE
HARDUIN-LEPERS ANNE
KURATA JUNICHI
AOKI-KINOSHITA KIYOKO F
SATO CHIHIRO
KHOO KAY-HOOI
KITAJIMA KEN
GUERARDEL YANN

RIVISTA

Nature Communications, 9, 4647

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