Pubblicazione scientifica

Pathogenic waterborne free-living amoebae: An update from selected Southeast Asian countries

FILONE TECNOLOGICO	TEMA
produzione e cattura

RIASSUNTO

Data on the distribution of free-living amoebae is still lacking especially in Southeast Asian region. The aquatic environment revealed a high occurrence of free-living amoebae (FLA) due to its suitable condition and availability of food source, which subsequently causes infection to humans. A total of 94 water samples consisted of both treated and untreated from Laos (31), Myanmar (42), and Singapore (21) were investigated for the presence of pathogenic FLA. Each water sample was filtered and cultured onto non-nutrient agar seeded with live suspension of Escherichia coli and incubated at room temperature. Morphological identification was conducted for both trophozoites and cysts via microscopic stains (Giemsa and immunofluorescence). The presence of Naegleria-like structures was the most frequently encountered in both treated and untreated water samples, followed by Acanthamoeba-like and Vermamoeba-like features. To identify the pathogenic isolates, species-specific primer sets were applied for molecular identification of Acanthamoeba, Naegleria, and Vermamoeba. The pathogenic species of Acanthamoeba lenticulata and A. triangularis were detected from untreated water samples, while Vermamoeba vermiformis was found in both treated and untreated water samples. Our results suggested that poor water quality as well as inadequate maintenance and treatment might be the cause of this alarming problem since chlorine disinfection is ineffective in eradicating these amoebas in treated water samples. Regular monitoring and examination of water qualities are necessary in order to control the growth, hence, further preventing the widespread of FLA infections among the public.

DATA

Data di pubblicazione: 17/02/2017

AUTORI

ABDUL MAJID MOHAMAD AZLAN
MAHBOOB TOOBA
MONG BRANDON G J
JATURAS NARONG
RICHARD REENA LEEBA
TIAN-CHYE TAN
PHIMPHILA ANUSORN
MAHAPHONH PANOMPHANH
AYE KYAW NYEIN
AUNG WAI LYNN
CHUAH JOON
ZIEGLER ALAN D
YASIRI ATIPAT
SAWANGJAROEN NONGYAO
LIM YVONNE A L
NISSAPATORN VEERANOOT

ENTE DI AFFERENZA

DEPT PARASITOLOGY SOUTHEAST ASIA WATER TEAM FACULTY OF MEDICINE UNIV MALAYA KUALA LUMPUR MALAYSIA
DEPT MEDICAL LAB FACULTY OF MEDICAL TECH UNIV HEALTH SCIENCES VIENTIANE LAOS PDR
ECOLOGICAL LAB
INST WATER POLICY NATL UNIV SINGAPORE SINGAPORE SINGAPORE
DEPT GEOGRAPHY NATL UNIV SINGAPORE SINGAPORE SINGAPORE
CHULABHORN INTL COLLEGE OF MEDICINE THAMMASAT UNIV PATHUM THANI THAILAND
DEPT MICROBIOLOGY FACULTY OF SCIENCE PRINCE OF SONGKHLA UNIV HAT YAI THAILAND
NATL CHENG KUNG UNIV TAIWAN

RIVISTA

PLoS ONE, 12(2), e0169448

IMPACT FACTOR

2,870

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