Pubblicazione scientifica

Attenuation of Vibrio parahaemolyticus Virulence Factors by a Mixture of Natural Antimicrobials

FILONE TECNOLOGICO	TEMA
trasformazione e commercializzazione

RIASSUNTO

Reducing acute mortality in aquatic crustaceans using natural alternatives to antibiotics has become a necessity, firstly for its positive impact on the aquaculture industry and, secondly, because the extensive use of antibiotics may lead to increased levels of drug resistance in pathogenic microorganisms. This study aimed to investigate the effect of a mixture of natural antimicrobials on the in vitro and in vivo virulence abilities of Type VI secretion system (T6SS)-positive Vibrio parahaemolyticus (A3 and D4), strains known as having potentially harmful health consequences for aquatic crustaceans and consumers. Herein, we report that a natural antimicrobial mixture (A3009) was capable of significantly reducing the virulence of V. parahaemolyticus strains A3 and D4 in an in vitro infection model, using the fish cell line CHSE-214, an effect which correlates with the bacterial downregulation of hcp1 and hcp2 gene expression and with the ability of the antimicrobial to efficiently retain low cytotoxic levels (p < 0.001). We show for the first time that a natural antimicrobial is able to significantly reduce the mortality of shrimps in a challenge experiment and is able to significantly attenuate H2O2 release during infection (p < 0.001), indicating that it could harbor positive intestinal redox balance effects.

DATA

Data di pubblicazione: 11/12/2019

AUTORI

PINKERTON LAURETTE
LINTON MARK
KELLY CARMEL
WARD PATRICK
GRADISTEANU PIRCALABIORU GRATIELA
PET IOAN
STEF LAVINIA
SIMA FILIP
ADAMOV TABITA
GUNDOGDU OZAN
CORCIONIVOSCHI NICOLAE

ENTE DI AFFERENZA

BACTERIOLOGY BRANCH VETERINARY SCIENCES DIVISION AGRI FOOD & BIOSCIENCES INST BELFAST BT9 5PX UK LAURETTE PINKERTON AFBINI GOV UK LP MARK LINTON AFBINI GOV UK ML CARMEL KELLY AFBINI GOV UK CK FILIP SIMA AFBINI GOV UK FS
AURANTA NOVAUCD DUBLIN 4 IRELAND PAT AURANTA IE
RESEARCH INST UNIV BUCHAREST 300645 BUCHAREST ROMANIA GRATIELA87 GMAIL COM
BANAT UNIV ANIMAL SCIENCES & VETERINARY MEDICINE KING MICHAEL I OF ROMANIA
LONDON SCHOOL OF HYGIENE & TROPICAL MEDICINE LONDON WC1E 7HT UK

RIVISTA

Microorganisms, 7(12), 679

IMPACT FACTOR

4,152

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