Pubblicazione scientifica

Generation of Stable Induced Pluripotent Stem-like Cells from Adult Zebra Fish Fibroblasts

FILONE TECNOLOGICO	TEMA
trasformazione e commercializzazione

RIASSUNTO

Induced pluripotent stem (iPS) cells provide a powerful platform for the study of development, regeneration, and disease. Although many stable iPS cell lines have been established for mammals, few attempts have been made to induce iPS cells in nonmammalian species. Because of technical advantages over other vertebrates on stem cells, induced pluripotent stem cells from fish could be of value for research. In this paper, stable iPS-like cell lines were generated from adult zebra fish fibroblasts by combining the doxycycline inducible lentiviral delivery system and chemical treatment. RT-PCR analysis, alkaline phosphatase staining, and immunofluorescence indicated that adult zebra fish fibroblasts were successfully reprogrammed into iPS-like cells (ziPSCs). The ziPSCs exhibited stable growth and manifested many features of fish embryonic stem cells with pluripotency in vitro and in vivo. Because of easy maintenance, the developed technology in this study for generating zebra fish iPS-like cells could be extended to investigating other genera of fish.

DATA

Data di pubblicazione: 24/08/2019

AUTORI

PENG LIANGYUE
ZHOU YONGHUA
XU WENTING
JIANG MINGGUI
LI HUAJIN
LONG MINDI
LIU WENBIN
LIU JINHUI
ZHAO XIAOYANG
XIAO YAMEI

ENTE DI AFFERENZA

STATE KEY LAB OF DEV BIOLOGY OF FRESHWATER FISH HUNAN NORMAL UNIV CHANGSHA 410081 CHINA
COLLEGE OF LIFE SCIENCES HUNAN NORMAL UNIV CHANGSHA HUNAN 410081 CHINA
COLLEGE OF BIOLOGY & ENVIRONMENTAL ENG CHANGSHA UNIV CHANGSHA 410081 CHINA
SCHOOL OF BASIC MEDICAL SCIENCES SOUTHERN MEDICAL UNIV GUANGZHOU 510515 CHINA

RIVISTA

International Journal of Biological Sciences, 15(11), 2340-2349

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