Pubblicazione scientifica

Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease

FILONE TECNOLOGICO	TEMA
trasformazione e commercializzazione

RIASSUNTO

Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.

DATA

Data di pubblicazione: 09/07/2019

AUTORI

MARSIAN JOHANNA
HURDISS DANIEL L
RANSON NEIL A
RITALA ANNELI
PALEY RICHARD
CANO IRENE
LOMONOSSOFF GEORGE P

ENTE DI AFFERENZA

JOHN INNES CTR NORWICH RSCH PARK NORWICH UK
ASTBURY CTR STRUCTURAL MOLECULAR BIOLOGY SCHOOL OF MOLECULAR & CELLULAR BIOLOGY UNIV LEEDS LEEDS UK
VTT TECH RSCH CTR FINLAND ESPOO FINLAND
CTR ENVIRONMENT FISHERIES & AQUACULTURE SCIENCE CEFAS WEYMOUTH LAB WEYMOUTH UK

RIVISTA

Frontiers in Plant Science, 10, 880

IMPACT FACTOR

4,300

indietro