Pubblicazione scientifica

Identification of sex chromosomes in Eremias velox ( Lacertidae, Reptilia) using lampbrush chromosome analysis

FILONE TECNOLOGICO	TEMA
trasformazione e commercializzazione

RIASSUNTO

Abstract

Reptiles are good objects for studying the evolution of sex determination, since they have different sex determination systems in different lineages. Lacertid lizards have been long-known for possessing ZZ/ZW type sex chromosomes. However, due to morphological uniformity of lacertid chromosomes, the Z chromosome has been only putatively cytologically identified. We used lampbrush chromosome ( LBC) analysis and FISH with a W-specific probe in Eremias velox (Pallas, 1771) to unequivocally identify the ZW bivalent and investigate its meiotic behavior. The heterochromatic W chromosome is decondensed at the lampbrush stage, indicating active transcription, contrast with the highly condensed condition of the lampbrush W chromosomes in birds. We identified the Z chromosome by its chiasmatic association with the W chromosome as chromosome XIII of the 19 chromosomes in the LBC karyotype. Our findings agree with previous genetic and genomic studies, which suggested that the lacertid Z chromosome should be one of the smaller macrochromosomes.

DATA

Data di pubblicazione: 14/05/2019

AUTORI

LISACHOV ARTEM P
GALKINA SVETLANA A
SAIFITDINOVA ALSU F
SVETLANA A ROMANENKO
ANDREYUSHKOVA DARIA A
TRIFONOV VLADIMIR A
BORODIN PAVEL M

ENTE DI AFFERENZA

INST CYTOLOGY & GENETICS SB RAS ACAD
SAINT PETERSBURG STATE UNIVERSITY UNIVERSITETSKAYA EMB 7–9 SAINT PETERSBURG 199034 RUSSIA
HERZEN STATE PEDAGOGICAL UNIV RUSSIA MOIKA EMB 48 SAINT
INST MOLECULAR & CELLULAR BIOLOGY SB RAS ACAD
NOVOSIBIRSK STATE UNIV

RIVISTA

Comparative Cytogenetics, 13(2), 121-132

IMPACT FACTOR

0,980

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