Pubblicazione scientifica

Efficient Construction and Effective Screening of Synthetic Domain Antibody Libraries

FILONE TECNOLOGICO	TEMA
trasformazione e commercializzazione

RIASSUNTO

Phage display is a powerful technique for drug discovery in biomedical research in particular for antibody libraries. But, several technical challenges are associated with the selection process. For instance, during the panning step, the successful elution of the phages bound to the antigen is critical in order to avoid losing the most promising binders. Here, we present an efficient protocol to establish, screen and select synthetic libraries of domain antibodies using phage display. We do not only present suitable solutions to the above-mentioned challenges to improve elution by 50-fold, but we also present a step by step in-depth protocol with miniaturized volumes and optimized procedures to save material, costs and time for a successful phage display with domain antibodies. Hence, this protocol improves the selection process for an efficient handling process. The here presented library is based on the variable domain (vNAR) of the naturally occurring novel antibody receptor (IgNAR) from cartilage fishes. Diversity was introduced in the Complementarity-Determining Region 3 (CDR3) of the antigen-binding site with different composition and length.

DATA

Data di pubblicazione: 14/02/2019

AUTORI

SOLEMANI ZADEH ARGHAVAN
GRASSER ALISSA
DINTER HEIKO
HERMES MAXIMILIAN
SCHINDOWSKI KATHARINA

ENTE DI AFFERENZA

INST APPLIED BIOTECHNOLOGY BIBERACH UNIV APPLIED SCIENCE HUBERTUS
FACULTY OF MEDICINE GRADUATE SCHOOL MOLECULAR MEDICINE UNIVERSITY OF ULM ALBERT-EINSTEIN-ALLEE 11 89081 ULM GERMANY
FACULTY OF NATURAL SCIENCES UNIV ULM ALBERT

RIVISTA

Methods and Protocols, 2(1), 17

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