Pubblicazione scientifica

Increased Chromosome Aberrations in Cells Exposed Simultaneously to Simulated Microgravity and Radiation

FILONE TECNOLOGICO	TEMA
trasformazione e commercializzazione

RIASSUNTO

Space radiation and microgravity (μG) are two major environmental stressors for humans in space travel. One of the fundamental questions in space biology research is whether the combined effects of μG and exposure to cosmic radiation are interactive. While studies addressing this question have been carried out for half a century in space or using simulated μG on the ground, the reported results are ambiguous. For the assessment and management of human health risks in future Moon and Mars missions, it is necessary to obtain more basic data on the molecular and cellular responses to the combined effects of radiation and µG. Recently we incorporated a μG–irradiation system consisting of a 3D clinostat synchronized to a carbon-ion or X-ray irradiation system. Our new experimental setup allows us to avoid stopping clinostat rotation during irradiation, which was required in all other previous experiments. Using this system, human fibroblasts were exposed to X-rays or carbon ions under the simulated μG condition, and chromosomes were collected with the premature chromosome condensation method in the first mitosis. Chromosome aberrations (CA) were quantified by the 3-color fluorescent in situ hybridization (FISH) method. Cells exposed to irradiation under the simulated μG condition showed a higher frequency of both simple and complex types of CA compared to cells irradiated under the static condition by either X-rays or carbon ions.

DATA

Data di pubblicazione: 22/12/2018

AUTORI

HADA MEGUMI
IKEDA HIROKO
RHONE JORDAN R
BEITMAN ANDREW J
PLANTE IANIK
SOUDA HIKARU
YOSHIDA YUKARI
HELD KATHRYN D
FUJIWARA KEIGI
SAGANTI PREMKUMAR B
TAKAHASHI AKIHISA

ENTE DI AFFERENZA

RADIATION INST SCIENCE & ENG PRAIRIE VIEW A & M UNIV PRAIRIE VIEW TX 77446 USA JRRHONE PVAMU EDU JRR AJBEITMAN PVAMU EDU AJB PBSAGANTI PVAMU EDU PBS
GUNMA UNIV INITIATIVE FOR ADV RSCH GUNMA UNIV MAEBASHI GUNMA 371 8511 JAPAN HI IKEDA GUNMA U AC JP HI KHELD MGH HARVARD EDU KDH
KBRWYLE
HEAVY ION MEDICAL CTR GUNMA UNIV MAEBASHI GUNMA 371 8511 JAPAN SOUDA GUNMA U AC JP HS YYUKARI GUNMA U AC JP YY A TAKAHASHI GUNMA U AC JP AT
DEPT RADIATION ONCOLOGY MASSACHUSETTS GEN HOSPITAL HARVARD MEDICAL SCHOOL BOSTON MA 02114 USA
DEPT CADIOLOGY UNIV TEXAS MD ANDERSON CANCER CTR HOUSTON TX 77030 USA KFUJIWARA1 MDANDERSON ORG

RIVISTA

International Journal of Molecular Sciences, 20(1), 43

IMPACT FACTOR

4,210

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