Pubblicazione scientifica

The Greenland shark Somniosus microcephalus—Hemoglobins and ligand-binding properties

FILONE TECNOLOGICO	TEMA
economia circolare e sostenibilità	monitoraggio impatti climatici sostenibilità dell'ecosistema marino
produzione e cattura	attrezzi da pesca cattura selettiva
trasformazione e commercializzazione	macchinari di ausilio alla lavorazione e trasformazione del pesce sistemi di trasporto, logistica e catena del freddo

RIASSUNTO

A large amount of data is currently available on the adaptive mechanisms of polar bony fish hemoglobins, but structural information on those of cartilaginous species is scarce. This study presents the first characterisation of the hemoglobin system of one of the longest-living vertebrate species (392 ± 120 years), the Arctic shark Somniosus microcephalus. Three major hemoglobins are found in its red blood cells and are made of two copies of the same α globin combined with two copies of three very similar β subunits. The three hemoglobins show very similar oxygenation and carbonylation properties, which are unaffected by urea, a very important compound in marine elasmobranch physiology. They display identical electronic absorption and resonance Raman spectra, indicating that their heme-pocket structures are identical or highly similar. The quaternary transition equilibrium between the relaxed (R) and the tense (T) states is more dependent on physiological allosteric effectors than in human hemoglobin, as also demonstrated in polar teleost hemoglobins. Similar to other cartilaginous fishes, we found no evidence for functional differentiation among the three isoforms. The very similar ligand-binding properties suggest that regulatory control of O2 transport may be at the cellular level and that it may involve changes in the cellular concentrations of allosteric effectors and/or variations of other systemic factors. The hemoglobins of this polar shark have evolved adaptive decreases in O2 affinity in comparison to temperate sharks.

DATA

Data di pubblicazione: 12/10/2017

AUTORI

RUSSO ROBERTA
GIORDANO DANIELA
PAREDI GIANLUCA
MARCHESANI FRANCESCO
MILAZZO LISA
ALTOMONTE GIOVANNA
DEL CANALE PIETRO
ABBRUZZETTI STEFANIA
ASCENZI PAOLO
DI PRISCO GUIDO
VIAPPIANI CRISTIANO
FAGO ANGELA
BRUNO STEFANO
SMULEVICH GIULIETTA
VERDE CINZIA

ENTE DI AFFERENZA

Dipartimento di Chimica “Ugo Schiff” Università di Firenze Via della Lastruccia 3–13 Sesto Fiorentino (FI) Italy
INST BIOSCIENCES & BIORESOURCES CNR
STAZIONE ZOOLOGICA ANTON DOHRN VILLA COMUNALE NAPLES ITALY
DIPARTIMENTO DI SCIENZE DEGLI ALIMENTI E DEL FARMACO UNIV DI PARMA PARCO AREA DELLE SCIENZE 23 A PARMA ITALY
DIPARTIMENTO DI BIOLOGIA UNIV ROMA
DIPARTIMENTO DI SCIENZE MATEMATICHE FISICHE E INFORMATICHE UNIV DEGLI STUDI DI PARMA PARCO AREA DELLE SCIENZE 7A PARMA ITALY
NEST INST NANOSCIENZE CNR PIAZZA SAN SILVESTRO 12 PISA ITALY
LAB INTERDIPARTIMENTALE DI MICROSCOPIA ELTNC UNIV ROMATRE
ZOOPHYSIOLOGY DEPT BIOSCIENCE AARHUS UNIV AARHUS DENMARK
JAMES COOK UNIV AUSTRALIA

RIVISTA

PLoS ONE, 12(10), e0186181

IMPACT FACTOR

2,870

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