Pubblicazione scientifica

Development and characterization of an oral multispecies biofilm implant flow chamber model

FILONE TECNOLOGICO	TEMA
trasformazione e commercializzazione

RIASSUNTO

Peri-implant infections are the most common cause of implant failure in modern dental implantology. These are caused by the formation of biofilms on the implant surface and consist of oral commensal and pathogenic bacteria, which harm adjacent soft and hard tissues and may ultimately lead to implant loss. In order to improve the clinical situation, there has to be a better understanding of biofilm formation on abiotic surfaces. Therefore, we successfully developed a system to cultivate an oral multispecies biofilm model in a flow chamber system, optimized for the evaluation of biofilm formation on solid materials by direct microscopic investigation. The model contains four relevant oral bacterial species: Streptococcus oralis, Actinomyces naeslundii, Veillonella dispar and Porphyromonas gingivalis in ratios similar to the native situation. The reliability of the developed “Hanoverian Oral Multispecies Biofilm Implant Flow Chamber” (HOBIC) model was verified. Biofilm volume and live/dead distribution within biofilms were determined by fluorescence staining and confocal laser scanning microcopy (CLSM). The individual species distribution was analyzed using quantitative real time PCR with propidium monoazide pretreatment (PMA-qRT-PCR) and by urea-NaCl fluorescence in situ hybridization (urea-NaCl-FISH). This in vitro model may be used to analyze biofilm formation on dental implants in more detail and to develop future implant systems with improved material properties.

DATA

Data di pubblicazione: 17/05/2018

AUTORI

KOMMEREIN NADINE
DOLL KATHARINA
STUMPP NICO S
STIESCH MEIKE

ENTE DI AFFERENZA

CLINIC FOR DENTAL PROSTHETICS & BIOMEDICAL MATERIALS SCIENCE HANNOVER MEDICAL SCHOOL HANNOVER GERMANY
OREGON HEALTH & SCIENCE UNIV US

RIVISTA

PLoS ONE, 13(5), e0196967

IMPACT FACTOR

2,870

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