Pubblicazione scientifica

Molecular cytogenetic (FISH) and genome analysis of diploid wheatgrasses and their phylogenetic relationship

FILONE TECNOLOGICO	TEMA
trasformazione e commercializzazione

RIASSUNTO

This paper reports detailed FISH-based karyotypes for three diploid wheatgrass species Agropyron cristatum (L.) Beauv., Thinopyrum bessarabicum (Savul.&Rayss) A. Löve, Pseudoroegneria spicata (Pursh) A. Löve, the supposed ancestors of hexaploid Thinopyrum intermedium (Host) Barkworth & D.R.Dewey, compiled using DNA repeats and comparative genome analysis based on COS markers. Fluorescence in situ hybridization (FISH) with repetitive DNA probes proved suitable for the identification of individual chromosomes in the diploid JJ, StSt and PP genomes. Of the seven microsatellite markers tested only the (GAA)n trinucleotide sequence was appropriate for use as a single chromosome marker for the P. spicata AS chromosome. Based on COS marker analysis, the phylogenetic relationship between diploid wheatgrasses and the hexaploid bread wheat genomes was established. These findings confirmed that the J and E genomes are in neighbouring clusters.

DATA

Data di pubblicazione: 09/03/2017

AUTORI

LINC GABRIELLA
GAAL ESZTER
MOLNAR ISTVAN
ICSO DIANA
BADAEVA EKATERINA
MOLNAR-LANG MARTA

ENTE DI AFFERENZA

DEPT PLANT GENETIC RESOURCES AGRICULTURAL INST CTR AGRICULTURAL RSCH HUNGARIAN ACAD SCIENCES MARTONVASAR HUNGARY
VAVILOV INST GEN GENETICS RUSSIAN ACAD SCIENCES MOSCOW RUSSIA
LEIBNIZ INST PLANT GENETICS & CROP PLANT RSCH IPK GERMANY

RIVISTA

PLoS ONE, 12(3), e0173623

IMPACT FACTOR

2,870

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