Pubblicazione scientifica

Expression and function of ATP-dependent potassium channels in zebrafish islet β-cells

FILONE TECNOLOGICO	TEMA
trasformazione e commercializzazione

RIASSUNTO

ATP-sensitive potassium channels (KATP channels) are critical nutrient sensors in many mammalian tissues. In the pancreas, KATP channels are essential for coupling glucose metabolism to insulin secretion. While orthologous genes for many components of metabolism–secretion coupling in mammals are present in lower vertebrates, their expression, functionality and ultimate impact on body glucose homeostasis are unclear. In this paper, we demonstrate that zebrafish islet β-cells express functional KATP channels of similar subunit composition, structure and metabolic sensitivity to their mammalian counterparts. We further show that pharmacological activation of native zebrafish KATP using diazoxide, a specific KATP channel opener, is sufficient to disturb glucose tolerance in adult zebrafish. That β-cell KATP channel expression and function are conserved between zebrafish and mammals illustrates the evolutionary conservation of islet metabolic sensing from fish to humans, and lends relevance to the use of zebrafish to model islet glucose sensing and diseases of membrane excitability such as neonatal diabetes.

DATA

Data di pubblicazione: 08/02/2017

AUTORI

EMFINGER CHRISTOPHER H
WELSCHER ALECIA
YAN ZIHAN
WANG YIXI
CONWAY HANNAH
MOSS JENNIFER B
MOSS LARRY G
REMEDI MARIA S
NICHOLS COLIN G

ENTE DI AFFERENZA

DEPT CELL BIOLOGY & PHYSIOLOGY WASHINGTON UNIV IN ST LOUIS ST LOUIS MO USA
ENDOCRINOLOGY METABOLISM & LIPID RSCH DEPT MEDICINE WASHINGTON UNIV IN ST LOUIS ST LOUIS MO USA
CTR INVESTIGATION OF MEMBRANE EXCITABILITY DISEASES WASHINGTON UNIV IN ST LOUIS ST LOUIS MO USA
ENDOCRINOLOGY METABOLISM & NUTRITION & DMPI DUKE UNIV MEDICAL CTR DURHAM NC USA

RIVISTA

Royal Society Open Science, 4(2), 160808

IMPACT FACTOR

2,680

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