Pubblicazione scientifica

Lipidomic analysis of Arabidopsis seed genetically engineered to contain DHA

FILONE TECNOLOGICO	TEMA
trasformazione e commercializzazione

RIASSUNTO

Metabolic engineering of omega-3 long-chain (≥C20) polyunsaturated fatty acids (ω3 LC-PUFA) in oilseeds has been one of the key targets in recent years. By expressing a transgenic pathway for enhancing the synthesis of the ω3 LC-PUFA docosahexaenoic acid (DHA) from endogenous α-linolenic acid (ALA), we obtained the production of fish oil-like proportions of DHA in Arabidopsis seed oil. Liquid chromatography-mass spectrometry (LC-MS) was used to characterize the triacylglycerol (TAG), diacylglycerol (DAG) and phospholipid (PL) lipid classes in the transgenic and wild type Arabidopsis seeds at both developing and mature stages. The analysis identified the appearance of several abundant DHA-containing phosphatidylcholine (PC), DAG and TAG molecular species in mature seeds. The relative abundances of PL, DAG, and TAG species showed a preferred combination of LC-PUFA with ALA in the transgenic seeds, where LC-PUFA were esterified in positions usually occupied by 20:1ω9. Trace amounts of di-DHA PC and tri-DHA TAG were identified and confirmed by high resolution MS/MS. Studying the lipidome in transgenic seeds provided insights into where DHA accumulated and combined with other fatty acids of neutral and phospholipids from the developing and mature seeds.

DATA

Data di pubblicazione: 01/09/2014

AUTORI

ZHOU XUE-RONG
CALLAHAN DAMIEN L
SHRESTHA PUSHKAR
LIU QING
PETRIE JAMES R
SINGH SURINDER P

ENTE DI AFFERENZA

FOOD FUTURES NATL RSCH FLAGSHIP COMMONWEALTH SCIENTIFIC & IND RSCH ORG CANBERRA ACT AUSTRALIA
PLANT IND COMMONWEALTH SCIENTIFIC & IND RSCH ORG CANBERRA ACT AUSTRALIA
METABOLOMICS AUSTRALIA SCHOOL OF BOTANY UNIV MELBOURNE MELBOURNE VIC AUSTRALIA
CTR CHEMISTRY & BIOTECHNOLOGY SCHOOL OF LIFE & ENVIRONMENTAL SCIENCES DEAKIN UNIV MELBOURNE VIC AUSTRALIA

RIVISTA

Frontiers in Plant Science, 5, 419

IMPACT FACTOR

4,300

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