Pubblicazione scientifica

Isolation and Characterization of Glycophorin from Carp Red Blood Cell Membranes

FILONE TECNOLOGICO	TEMA
trasformazione e commercializzazione

RIASSUNTO

We isolated a high-purity carp glycophorin from carp erythrocyte membranes following extraction using the lithium diiodosalicylate (LIS)-phenol method and streptomycin treatment. The main carp glycophorin was observed to locate at the position of the carp and human band-3 proteins on an SDS-polyacrylamide gel. Only the N-glycolylneuraminic acid (NeuGc) form of sialic acid was detected in the carp glycophorin. The oligosaccharide fraction was separated into two components (P-1 and P-2) using a Glyco-Pak DEAE column. We observed bacteriostatic activity against five strains of bacteria, including two known fish pathogens. Fractions from the carp erythrocyte membrane, the glycophorin oligosaccharide and the P-1 also exhibited bacteriostatic activity; whereas the glycolipid fraction and the glycophorin fraction without sialic acid did not show the activity. The carp glycophorin molecules attach to the flagellum of V. anguillarum or the cell surface of M. luteus and inhibited bacterial growth.

DATA

Data di pubblicazione: 08/08/2014

AUTORI

AOKI TAKAHIKO
CHIMURA KENJI
NAKAO NOBUHIRO
MIZUNO YASUKO

ENTE DI AFFERENZA

LAB OF QUALITY IN MARINE PROD GRADUATE SCHOOL OF BIORESOURCES MIE UNIV
TORAY RSCH CTR INC

RIVISTA

Membranes, 4(3), 491-508

IMPACT FACTOR

3,180

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