Pubblicazione scientifica

Soluble Fas and the −670 Polymorphism of Fas in Lupus Nephritis

FILONE TECNOLOGICO	TEMA
trasformazione e commercializzazione

RIASSUNTO

This study was performed to clarify the role of soluble Fas (sFas) in lupus nephritis (LN) and establish a potential relationship between LN and the −670 polymorphism of Fas in 67 patients with systemic lupus erythematosus (SLE), including a subset of 24 LN patients with proteinuria. Additionally, a group of 54 healthy subjects (HS) was included. The allelic frequency of the −670 polymorphism of Fas was determined using PCR-RFLP analysis, and sFas levels were assessed by ELISA. Additionally, the WT-1 protein level in urine was measured. The Fas receptor was determined in biopsies by immunohistochemistry (IHC) and in situ hybridization (FISH) and apoptotic features by TUNEL. Results. The −670 Fas polymorphism showed that the G allele was associated with increased SLE susceptibility, with an odds ratio (OR) of 1.86. The sFas was significantly higher in LN patients with the G/G genotype, and this subgroup exhibited correlations between the sFas level and proteinuria and increased urinary WT-1 levels. LN group shows increased expression of Fas and apoptotic features. In conclusion, our results indicate that the G allele of the −670 polymorphism of Fas is associated with genetic susceptibility in SLE patients with elevated levels of sFas in LN with proteinuria.

DATA

Data di pubblicazione: 01/01/2014

AUTORI

BOLLAIN-Y-GOYTIA JUAN JOSE
ARELLANO-RODRIGUEZ MARIELA
TORRES-DEL-MURO FELIPE DE JESUS
DAZA-BENITEZ LEONEL
FRANCISCO MUNOZ-VALLE JOSE
AVALOS-DIAZ ESPERANZA
HERRERA-ESPARZA RAFAEL

ENTE DI AFFERENZA

LAB DE INMUNOLOGIA Y BIOLOGIA MOLECULAR UA CIENCIAS BIOLOGICAS UNIV AUTONOMA DE ZACATECAS 98040 ZACATECAS ZAC MEXICO
UNIDAD MEDICA DE ALTA ESPECIALIDAD UMAE HPG NO 48 INST MEXICANO DEL SEGURO SOCIAL IMSS 37320 LEON GTO MEXICO
INST INVESTIGACION EN CIENCIAS BIOMEDICAS CTR UNIVERSITARIO DE CIENCIAS SALUD UNIV GUADALAJARA 45178 GUADALAJARA JAL MEXICO

RIVISTA

International Journal of Nephrology, 2014, 780406

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