Pubblicazione scientifica

Prokaryotic Diversity in Aran-Bidgol Salt Lake, the Largest Hypersaline Playa in Iran

FILONE TECNOLOGICO	TEMA
trasformazione e commercializzazione

RIASSUNTO

Prokaryotic diversity in Aran-Bidgol salt lake, a thalasohaline lake in Iran, was studied by fluorescence in situ hybridization (FISH), cultivation techniques, denaturing gradient gel electrophoresis (DGGE) of PCR-amplified fragments of 16S rRNA genes and 16S rRNA gene clone library analysis. Viable counts obtained (2.5–4 × 106 cells mL−1) were similar to total cell abundance in the lake determined by DAPI direct count (3–4×107 cells mL−1). The proportion of Bacteria to Archaea in the community detectable by FISH was unexpectedly high and ranged between 1:3 and 1:2. We analyzed 101 archaeal isolates and found that most belonged to the genera Halorubrum (55%) and Haloarcula (18%). Eleven bacterial isolates obtained in pure culture were affiliated with the genera Salinibacter (18.7%), Salicola (18.7%) and Rhodovibrio (35.3%). Analysis of inserts of 100 clones from the eight 16S rRNA clone libraries constructed revealed 37 OTUs. The majority (63%) of these sequences were not related to any previously identified taxa. Within this sampling effort we most frequently retrieved phylotypes related to Halorhabdus (16% of archaeal sequences obtained) and Salinibacter (36% of bacterial sequences obtained). Other prokaryotic groups that were abundant included representatives of Haloquadratum, the anaerobic genera Halanaerobium and Halocella, purple sulfur bacteria of the genus Halorhodospira and Cyanobacteria.

DATA

Data di pubblicazione: 15/12/2011

AUTORI

MAKHDOUMI-KAKHKI ALI
AMOOZEGAR MOHAMMAD ALI
KAZEMI BAHRAM
PASIC LEJLA
VENTOSA ANTONIO

ENTE DI AFFERENZA

EXTREMOPHILE LABORATORY DEPARTMENT OF MICROBIOLOGY SCHOOL OF BIOLOGY COLLEGE OF SCIENCE UNIVERSITY OF TEHRAN 14155–6455 QODS TEHRAN IRAN
CELLULAR & MOLECULAR BIOLOGY RSCH CTR SHAHEED BEHESHTI UNIV MEDICAL SCIENCES TEHRAN IRAN
DEPARTMENT OF BIOLOGY UNIVERSITY OF LJUBLJANA VECNA POT 111 1000 LJUBLJANA SLOVENIA
DEPT MICROBIOLOGY & PARASITOLOGY FACULTY OF PHARMACY UNIV SEVILLA 41012 SEVILLA SPAIN

RIVISTA

Microbes and Environments, 27(1), 87-93

IMPACT FACTOR

2,390

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