Pubblicazione scientifica

Expression of LamB Vaccine Antigen in Wolffia globosa (Duck Weed) Against Fish Vibriosis

FILONE TECNOLOGICO	TEMA
economia circolare e sostenibilità

RIASSUNTO

Vibriosis is a commonly found bacterial disease identified among fish and shellfish cultured in saline waters. A multitude of Vibrio species have been identified as the causative agents. LamB, a member of outer membrane protein (OMPs) family of these bacteria is conserved among all Vibrio species and has been identified as an efficient vaccine candidate against vibriosis. Rootless duckweed (Wolffia) is a tiny, edible aquatic plant possessing characteristics suitable for the utilization as a bioreactor. Thus, we attempted to express a protective edible vaccine antigen against fish vibriosis in nuclear-transformed Wolffia. We amplified LamB gene from virulent Vibrio alginolyticus and it was modified to maximize the protein expression level and translocate the protein to the endoplasmic reticulum (ER) in plants. It was cloned into binary vector pMYC under the control of CaMV 35S promoter and introduced into Wolffia globosa by Agrobacterium-mediated transformation. Integration and expression of the LamB gene was confirmed by genomic PCR and RT-PCR. Western blot analysis revealed accumulation of the LamB protein in 8 transgenic lines. The cross-protective property of transgenic Wolffia was evaluated by orally vaccinating zebrafish through feeding fresh transgenic Wolffia and subsequently challenging with virulent V. alginolyticus. High relative percent survival (RPS) of the vaccinated fish (63.3%) confirmed that fish immunized with transgenic Wolffia were well-protected from Vibrio infection. These findings suggest that Wolffia expressed LamB could serve as an edible plant-based candidate vaccine model for fish vibriosis and feasibility of utilizing Wolffia as bioreactor to produce edible vaccines.

DATA

Data di pubblicazione: 20/08/2020

AUTORI

HEENATIGALA P P M
SUN ZUOLIANG
YANG JINGJING
ZHAO XUYAO
HOU HONGWEI

ENTE DI AFFERENZA

UNIV CHINESE ACAD SCIENCES BEIJING CHINA
STATE KEY LAB OF FRESHWATER ECOLOGY & BIOTECHNOLOGY KEY LAB OF AQUATIC BIODIVERSITY & CONSERVATION OF CHINESE ACAD SCIENCES INST HYDROBIOLOGY CHINESE ACAD SCIENCES WUHAN CHINA
INLAND AQUATIC RESOURCES & AQUACULTURE DIVISION IARAD NATL AQUATIC RESOURCES RSCH & DEV AGENCY NARA COLOMBO SRI LANKA

RIVISTA

Frontiers in Immunology, 11, 1857

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