Pubblicazione scientifica

Unusual Antioxidant Properties of 26S Proteasome Isolated from Cold-Adapted Organisms

FILONE TECNOLOGICO	TEMA
trasformazione e commercializzazione	certificazione e marchi di qualità macchinari di ausilio alla lavorazione e trasformazione del pesce sistemi di trasporto, logistica e catena del freddo

RIASSUNTO

The oxidative challenge represents an important factor affecting the adaptive strategies in Antarctic fish, but their impact on the protein degradation machinery still remains unclear. The previous analysis of the first 26S proteasome from the Antarctic red-blooded fish Trematomus bernacchii, evidenced improved antioxidant functions necessary to counteract the environmental pro-oxidant conditions. The purpose of this work was to carry out a study on 26S proteasomes from the temperate red-blooded Dicenthrarcus labrax and the icefish Chionodraco hamatus in comparison with the isoform already described from T. bernacchii, to better elucidate the cold-adapted physiological functions of this complex. Therefore, the 26S isoforms were isolated and the complementary DNAs (cDNAs) codifying the catalytic subunits were cloned. The biochemical characterization of Antarctic 26S proteasomes revealed their significantly higher structural stability and resistance to H2O2 with respect to that of the temperate counterpart, as also suggested by a comparative modeling analysis of the catalytic subunits. Moreover, in contrast to that observed in T. bernacchii, the 26S systems from C. hamatus and D. labrax were incapable to hydrolyze oxidized proteins in a ubiquitin-independent manner. Therefore, the ‘uncommon’ properties displayed by the Antarctic 26S proteasomes can mirror the impact exercised by evolutionary pressure in response to richly oxygenated environments.

DATA

Data di pubblicazione: 25/07/2017

AUTORI

GOGLIETTINO MARTA
COCCA ENNIO
FUSCO CARMELA
AGRILLO BRUNA
RICCIO ALESSIA
BALESTRIERI MARCO
FACCHIANO ANGELO
PEPE ANTONIO
PALMIERI GIANNA

ENTE DI AFFERENZA

INST BIOSCIENCES & BIORESOURCES NATL RSCH COUNCIL CNR IBBR
INST FOOD SCIENCES NATL RSCH COUNCIL CNR ISA

RIVISTA

International Journal of Molecular Sciences, 18(8), 1605

IMPACT FACTOR

4,210

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