Pubblicazione scientifica

Osmoregulated Chloride Currents in Hemocytes from Mytilus galloprovincialis

FILONE TECNOLOGICO	TEMA
economia circolare e sostenibilità	geolocalizzazione monitoraggio impatti climatici
trasformazione e commercializzazione	macchinari di ausilio alla lavorazione e trasformazione del pesce

RIASSUNTO

We investigated the biophysical properties of the transport mediated by ion channels in hemocytes from the hemolymph of the bivalve Mytilus galloprovincialis. Besides other transporters, mytilus hemocytes possess a specialized channel sensitive to the osmotic pressure with functional properties similar to those of other transport proteins present in vertebrates. As chloride fluxes may play an important role in the regulation of cell volume in case of modifications of the ionic composition of the external medium, we focused our attention on an inwardly-rectifying voltage-dependent, chloride-selective channel activated by negative membrane potentials and potentiated by the low osmolality of the external solution. The chloride channel was slightly inhibited by micromolar concentrations of zinc chloride in the bath solution, while the antifouling agent zinc pyrithione did not affect the channel conductance at all. This is the first direct electrophysiological characterization of a functional ion channel in ancestral immunocytes of mytilus, which may bring a contribution to the understanding of the response of bivalves to salt and contaminant stresses.

DATA

Data di pubblicazione: 09/12/2016

AUTORI

BREGANTE MONICA
CARPANETO ARMANDO
PIAZZA VERONICA
SBRANA FRANCESCA
VASSALLI MASSIMO
FAIMALI MARCO
GAMBALE FRANCO

ENTE DI AFFERENZA

INST BIOPHYSICS NATL RSCH COUNCIL OF ITALY IBF GENOVA ITALY
INST MARINE SCIENCES NATL RSCH COUNCIL OF ITALY ISMAR GENOVA ITALY
TOKAI UNIV JAPAN

RIVISTA

PLoS ONE, 11(12), e0167972

IMPACT FACTOR

2,870

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