Pubblicazione scientifica

Utilization of Crab Waste for Cost-Effective Bioproduction of Prodigiosin

FILONE TECNOLOGICO	TEMA
economia circolare e sostenibilità

RIASSUNTO

This study aimed to establish the culture process for the cost-effective production of prodigiosin (PG) from demineralized crab shell powder (de-CSP), a fishery processing byproduct created via fermentation. Among the tested PG-producing strains, Serratia marcescens TNU02 was demonstrated to be the most active strain. Various ratios of protein/de-CSP were used as the sources of C/N for PG biosynthesis. The PG yield was significantly enhanced when the casein/de-CSP ratio was controlled in the range of 3/7 to 4/6. TNU02 produced PG with a high yield (5100 mg/L) in a 15 L bioreactor system containing 4.5 L of a newly-designed liquid medium containing 1.6% C/N source (protein/de-CSP ratio of 3/7), 0.02% (NH4)2SO4, 0.1% K2HPO4, and an initial pH of 6.15, at 27 °C for 8 h in dark conditions. The red pigment was purified from the culture broth and then quantified as being PG by specific Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) and UV spectra analysis. The purified PG demonstrated moderate antioxidant and effective inhibition against four cancerous cell lines. Notably, this study was the first to report on using crab wastes for PG bioproduction with high-level productivity (5100 mg/L) in a large scale (4.5 L per pilot) in a short period of fermentation time (8 h). The salt compositions, including (NH4)2SO4 and K2HPO4, were also a novel finding for the enhancement of PG yield by S. marcescens in this report.

DATA

Data di pubblicazione: 22/10/2020

AUTORI

NGUYEN VAN BON
NGUYEN DAI NAM
NGUYEN ANH DZUNG
NGO VAN ANH
TON THAT QUANG
DOAN CHIEN THANG
PHAM THI PHUONG
TRAN THI PHUONG HANH
WANG SAN-LANG

ENTE DI AFFERENZA

INST RSCH & DEV DUY TAN UNIV DANANG 550000 VIETNAM NGUYENVANBON2 DUYTAN EDU VN
INST BIOTECHNOLOGY & ENVIRONMENT TAY NGUYEN UNIV BUON MA THUOT 630000 VIETNAM DAINAMNGUYEN EDU GMAIL COM DNN NADZUNGTAYNGUYENUNI YAHOO COM VN ADN NGOVANANH3979 GMAIL COM VAN
FACULTY OF CHEMISTRY UNIV SCIENCE HO CHI MINH CITY 700000 VIETNAM TTQUANG HCMUS EDU VN
VIETNAM NATL UNIV HO CHI MINH CITY 700000 VIETNAM
DEPT SCIENCE & TECH TAY NGUYEN UNIV BUON MA THUOT 630000 VIETNAM DCTHANG TTN EDU VN CTD PTPHUONG TTN EDU VN TPP TTPHANH TTN EDU VN TPHT
DEPT CHEMISTRY TAMKANG UNIV NEW TAIPEI CITY 25137 TAIWAN
LIFE SCIENCE DEV CTR TAMKANG UNIV NEW TAIPEI CITY 25137 TAIWAN

RIVISTA

Marine Drugs, 18(11), 523

IMPACT FACTOR

3,810

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