Pubblicazione scientifica

Magnetic Nanoparticles Fishing for Biomarkers in Artificial Saliva

FILONE TECNOLOGICO	TEMA
economia circolare e sostenibilità

RIASSUNTO

Magnetic nanoparticles (MNPs) were synthesized using the colloidal co-precipitation method and further coated with silica using the Stöber process. These were functionalized with carboxylic and amine functionalities for further covalent immobilization of antibodies on these MNPs. The procedure for covalent immobilization of antibodies on MNPs was developed using 1-ethyl-3-(dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The evaluation of the efficiency of the coupling reaction was carried out by UV-vis spectrophotometry. The developed antibodies coupled to MNPs were tested for the pre-concentration of two biomarkers tumor necrosis factor alpha (TNF-α) and Interleukin-10 (IL-10). Both biomarkers were assessed in the matrix based on phosphate-buffered saline solution (PBS) and artificial saliva (AS) to carry out the demonstration of the format assay. Supernatants were used to determine the number of free biomarkers for both studies. Reduction of the nonspecific saliva protein adsorption on the surface of the complex antibodies-MNPs to levels low enough to allow the detection of biomarkers in complex media has been achieved.

DATA

Data di pubblicazione: 31/08/2020

AUTORI

SAHA ARPITA
BEN HALIMA HAMDI
SAINI ABHISHEK
GALLARDO-GONZALEZ JUAN
ZINE NADIA
VINAS CLARA
ELAISSARI ABDELHAMID
ERRACHID ABDELHAMID
TEIXIDOR FRANCESC

ENTE DI AFFERENZA

INORGANIC MATERIALS LAB INST CIENCIA DE MATERIALS DE BARCELONA ICMAB CSIC CAMPUS UAB 08193 BELLATERRA SPAIN ARPITASAHA666 GMAIL COM AS AB76779 GMAIL COM AS CLARA ICMAB ES CV
MICRO & NANOBIOTECHNOLOGY LAB
LAGEPP UMR 5007 CNRS UNIV CLAUDE BERNARD LYON 1 UNIV LYON F 69622 LYON FRANCE ABDELHAMID ELAISSARI UNIV LYON1 FR

RIVISTA

Molecules, 25(17), 3968

IMPACT FACTOR

3,010

indietro