Pubblicazione scientifica

Chemically-Mediated Interactions Between Macroalgae, Their Fungal Endophytes, and Protistan Pathogens

FILONE TECNOLOGICO	TEMA
produzione e raccolta

RIASSUNTO

Filamentous fungi asymptomatically colonize the inner tissues of macroalgae, yet their ecological roles remain largely underexplored. Here, we tested if metabolites produced by fungal endophytes might protect their host against a phylogenetically broad spectrum of protistan pathogens. Accordingly, the cultivable fungal endophytes of four brown algal species were isolated and identified based on LSU and SSU sequencing. The fungal metabolomes were tested for their ability to reduce the infection by protistan pathogens in the algal model Ectocarpus siliculosus. The most active metabolomes effective against the oomycetes Eurychasma dicksonii and Anisolpidium ectocarpii, and the phytomixid Maullinia ectocarpii were further characterized chemically. Several pyrenocines isolated from Phaeosphaeria sp. AN596H efficiently inhibited the infection by all abovementioned pathogens. Strikingly, these compounds also inhibited the infection of nori (Pyropia yezoensis) against its two most devastating oomycete pathogens, Olpidiopsis pyropiae, and Pythium porphyrae. We thus demonstrate that fungal endophytes associated with brown algae produce bioactive metabolites which might confer protection against pathogen infection. These results highlight the potential of metabolites to finely-tune the outcome of molecular interactions between algae, their endophytes, and protistan pathogens. This also provide proof-of-concept toward the applicability of such metabolites in marine aquaculture to control otherwise untreatable diseases.

DATA

Data di pubblicazione: 21/12/2018

AUTORI

VALLET MARINE
STRITTMATTER MARTINA
MURUA PEDRO
LACOSTE SANDRINE
DUPONT JOELLE
HUBAS CEDRIC
GENTA-JOUVE GREGORY
GACHON CLAIRE M M
KIM GWANG HOON
PRADO SOIZIC

ENTE DI AFFERENZA

MUSEUM NATL D HISTOIRE NATURELLE UNITE MOLECULES DE COMM & ADAPTATION DES MICRO ORGANISMES UMR 7245 CP 54 PARIS FRANCE
SCOTTISH ASSOC MARINE SCIENCE SCOTTISH MARINE INST OBAN UK
INST SYSTEMATIQUE EVOLUTION BIODIVERSITE ISYEB MUSEUM NATL D HISTOIRE NATURELLE CNRS SORBONNE UNIV EPHE PARIS FRANCE
UNITE BIOLOGIE DES ORGANISMES & ECOSYSTEMES AQUATIQUES UMR BOREA MUSEUM NATL D HISTOIRE NATURELLE SORBONNE UNIV UNIV CAEN NORMANDIE UNIV ANTILLES CNRS IRD STATION MARINE DE CONCARNEAU CONCARNEAU FRANCE
UNIV PARIS DESCARTES LAB DE CHIM TOXICOLOGIE ANALYTIQUE & CELLULAIRE C TAC UMR CNRS 8638 COMETE PARIS FRANCE
DEPT BIOLOGY KONGJU NATL UNIV KONGJU SOUTH KOREA

RIVISTA

Frontiers in Microbiology, 9, 3161

IMPACT FACTOR

4,190

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