Pubblicazione scientifica

Genes regulating gland development in the cotton plant

FILONE TECNOLOGICO	TEMA
produzione e raccolta

RIASSUNTO

Summary

In seeds and other parts of cultivated, tetraploid cotton (Gossypium hirsutum L.), multicellular groups of cells lysigenously form dark glands containing toxic terpenoids such as gossypol that defend the plant against pests and pathogens. Using RNA‐seq analysis of embryos from near‐isogenic glanded (Gl2Gl2Gl3Gl3) versus glandless (gl2gl2gl3gl3) plants, we identified 33 genes that expressed exclusively or at higher levels in embryos just prior to gland formation in glanded plants. Virus‐induced gene silencing against three gene pairs led to significant reductions in the number of glands in the leaves, and significantly lower levels of gossypol and related terpenoids. These genes encode transcription factors and have been designated the ‘Cotton Gland Formation’ (CGF) genes. No sequence differences were found between glanded and glandless cotton for CGF1 and CGF2 gene pairs. The glandless cotton has a transposon insertion within the coding sequence of the Go PGF (synonym CGF3) gene of the A subgenome and extensive mutations in the promoter of D subgenome homeolog. Overexpression of Go PGF (synonym CGF3) led to a dramatic increase in gossypol and related terpenoids in cultured cells, whereas CRISPR/Cas9 knockout of Go PGF (synonym CGF3) genes resulted in glandless phenotype. Taken collectively, the results show that the Go PGF (synonym CGF3) gene plays a critical role in the formation of glands in the cotton plant. Seed‐specific silencing of CGF genes, either individually or in combination, could eliminate glands, thus gossypol, from the cottonseed to render it safe as food or feed for monogastrics.

DATA

Data di pubblicazione: 21/12/2018

AUTORI

JANGA MADHUSUDHANA R
PANDEYA DEVENDRA
CAMPBELL LEANNE M
KONGANTI KRANTI
VILLAFUERTE STEPHANY TOINGA
PUCKHABER LORRAINE
PEPPER ALAN
STIPANOVIC ROBERT D
SCHEFFLER JODI A
RATHORE KEERTI S

ENTE DI AFFERENZA

Southern Plains Agricultural Research Center USDA‐ARS College Station TX USA
Crop Genetics Research Unit USDA‐ARS Stoneville MS USA
INST PLANT GENOMICS & BIOTECHNOLOGY TEXAS A & M UNIV COLLEGE STATION TX USA
TEXAS A & M INST GENOME SCIENCES & SOCIETY TEXAS A & M UNIV COLLEGE STATION TX USA
DEPT BIOLOGY TEXAS A & M UNIV COLLEGE STATION TX USA
DEPT SOIL & CROP SCIENCES TEXAS A & M UNIV COLLEGE STATION TX USA

RIVISTA

Plant Biotechnology Journal, 17(6), 1142-1153

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