Pubblicazione scientifica

Characterization of the Escherichia coli Virulent Myophage ST32

FILONE TECNOLOGICO	TEMA
produzione e raccolta

RIASSUNTO

The virulent phage ST32 that infects the Escherichiacoli strain ST130 was isolated from a wastewater sample in China and analyzed. Morphological observations showed that phage ST32 belongs to the Myoviridae family, as it has an icosahedral capsid and long contractile tail. Host range analysis showed that it exhibits a broad range of hosts including non-pathogenic and pathogenic E. coli strains. Interestingly, phage ST32 had a much larger burst size when amplified at 20 °C as compared to 30 °C or 37 °C. Its double-stranded DNA genome was sequenced and found to contain 53,092 bp with a GC content of 44.14%. Seventy-nine open reading frames (ORFs) were identified and annotated as well as a tRNA-Arg. Only nineteen ORFs were assigned putative functions. A phylogenetic tree using the large terminase subunit revealed a close relatedness with four unclassified Myoviridae phages. A comparative genomic analysis of these phages showed that the Enterobacteria phage phiEcoM-GJ1 is the closest relative to ST32 and shares the same new branch in the phylogenetic tree. Still, these two phages share only 47 of 79 ORFs with more than 90% identity. Phage ST32 has unique characteristics that make it a potential biological control agent under specific conditions.

DATA

Data di pubblicazione: 07/11/2018

AUTORI

LIU HONGHUI
GEAGEA HANY
ROUSSEAU GENEVIEVE M
LABRIE SIMON J
TREMBLAY DENISE M
LIU XINCHUN
MOINEAU SYLVAIN

ENTE DI AFFERENZA

Félix d’Hérelle Reference Center for Bacterial Viruses Faculté de médecine dentaire Université Laval Québec City QC G1V 0A6 Canada
COLLEGE OF RESOURCES & ENVIRONMENT UNIV CHINESE ACAD SCIENCES BEIJING 101408 CHINA HONGHUILIU HOTMAIL COM
DEPT BIOCHIMIE DE MICROBIOLOGIE & BIO INFORMATIQUE FACULTE DES SCIENCES & GENIE UNIV LAVAL QUEBEC CITY QC G1V 0A6 CANADA HANY GEAGEA 1 ULAVAL CA HG GENEVIEVE ROUSSEAU GREB ULAVAL CA GMR SIMON LABRIE SYNTBIOLAB COM SJL DENISE TREMBLAY GREB ULAVAL CA DMT

RIVISTA

Viruses, 10(11), 616

IMPACT FACTOR

3,760

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