Pubblicazione scientifica

Structure of the Macrobrachium rosenbergii nodavirus: A new genus within the Nodaviridae?

FILONE TECNOLOGICO	TEMA
produzione e raccolta

RIASSUNTO

Macrobrachium rosenbergii nodavirus (MrNV) is a pathogen of freshwater prawns that poses a threat to food security and causes significant economic losses in the aquaculture industries of many developing nations. A detailed understanding of the MrNV virion structure will inform the development of strategies to control outbreaks. The MrNV capsid has also been engineered to display heterologous antigens, and thus knowledge of its atomic resolution structure will benefit efforts to develop tools based on this platform. Here, we present an atomic-resolution model of the MrNV capsid protein (CP), calculated by cryogenic electron microscopy (cryoEM) of MrNV virus-like particles (VLPs) produced in insect cells, and three-dimensional (3D) image reconstruction at 3.3 Å resolution. CryoEM of MrNV virions purified from infected freshwater prawn post-larvae yielded a 6.6 Å resolution structure, confirming the biological relevance of the VLP structure. Our data revealed that unlike other known nodavirus structures, which have been shown to assemble capsids having trimeric spikes, MrNV assembles a T = 3 capsid with dimeric spikes. We also found a number of surprising similarities between the MrNV capsid structure and that of the Tombusviridae: 1) an extensive network of N-terminal arms (NTAs) lines the capsid interior, forming long-range interactions to lace together asymmetric units; 2) the capsid shell is stabilised by 3 pairs of Ca2+ ions in each asymmetric unit; 3) the protruding spike domain exhibits a very similar fold to that seen in the spikes of the tombusviruses. These structural similarities raise questions concerning the taxonomic classification of MrNV.

DATA

Data di pubblicazione: 22/10/2018

AUTORI

HO KOK LIAN
GABRIELSEN MADS
BEH POAY LING
KUEH CHARE LI
THONG QIU XIAN
STREETLEY JAMES
TAN WEN SIANG
BHELLA DAVID

ENTE DI AFFERENZA

DEPT PATHOLOGY FACULTY OF MEDICINE & HEALTH SCIENCES UNIV PUTRA MALAYSIA UPM SERDANG SELANGOR MALAYSIA
CRUK BEATSON INST GARSCUBE CAMPUS GLASGOW SCOTLAND UK
DEPT MICROBIOLOGY FACULTY OF BIOTECHNOLOGY & BIOMOLECULAR SCIENCES UNIV PUTRA MALAYSIA UPM SERDANG SELANGOR MALAYSIA
MRC UNIV GLASGOW CTR VIRUS RSCH GARSCUBE CAMPUS GLASGOW SCOTLAND UK
INST BIOSCIENCE UNIV PUTRA MALAYSIA UPM SERDANG SELANGOR MALAYSIA
UNIV PITTSBURGH US

RIVISTA

PLoS Biology, 16(10), e3000038

IMPACT FACTOR

7,620

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