Pubblicazione scientifica

The Arctic charr (Salvelinus alpinus) genome and transcriptome assembly

FILONE TECNOLOGICO	TEMA
produzione e raccolta
trasformazione e commercializzazione

RIASSUNTO

Arctic charr have a circumpolar distribution, persevere under extreme environmental conditions, and reach ages unknown to most other salmonids. The Salvelinus genus is primarily composed of species with genomes that are structured more like the ancestral salmonid genome than most Oncorhynchus and Salmo species of sister genera. It is thought that this aspect of the genome may be important for local adaptation (due to increased recombination) and anadromy (the migration of fish from saltwater to freshwater). In this study, we describe the generation of a new genetic map, the sequencing and assembly of the Arctic charr genome (GenBank accession: GCF_002910315.2) using the newly created genetic map and a previous genetic map, and present several analyses of the Arctic charr genes and genome assembly. The newly generated genetic map consists of 8,574 unique genetic markers and is similar to previous genetic maps with the exception of three major structural differences. The N50, identified BUSCOs, repetitive DNA content, and total size of the Arctic charr assembled genome are all comparable to other assembled salmonid genomes. An analysis to identify orthologous genes revealed that a large number of orthologs could be identified between salmonids and many appear to have highly conserved gene expression profiles between species. Comparing orthologous gene expression profiles may give us a better insight into which genes are more likely to influence species specific phenotypes.

DATA

Data di pubblicazione: 13/09/2018

AUTORI

CHRISTENSEN KRIS A
RONDEAU ERIC B
MINKLEY DAVID R
LEONG JONG S
NUGENT CAMERON M
DANZMANN ROY G
FERGUSON MOIRA M
STADNIK AGNIESZKA
DEVLIN ROBERT H
MUZZERALL ROBIN
EDWARDS MICHAEL
DAVIDSON WILLIAM S
KOOP BEN F

ENTE DI AFFERENZA

FISHERIES & OCEANS CANADA CTR AQUACULTURE & ENVIRONMENTAL RSCH WEST VANCOUVER BRITISH COLUMBIA CANADA
UNIV VICTORIA DEPT BIOLOGY VICTORIA BRITISH COLUMBIA CANADA
SIMON FRASER UNIV MOLECULAR BIOLOGY & BIOCHEMISTRY BURNABY BRITISH COLUMBIA CANADA
UNIV GUELPH DEPT INTEGRATIVE BIOLOGY GUELPH ONTARIO CANADA
ICY WATERS LTD
UNIV ICELAND ICELAND

RIVISTA

PLoS ONE, 13(9), e0204076

IMPACT FACTOR

2,870

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