Pubblicazione scientifica

Cell entry of a host-targeting protein of oomycetes requires gp96

FILONE TECNOLOGICO	TEMA
economia circolare e sostenibilità
produzione e raccolta
trasformazione e commercializzazione

RIASSUNTO

The animal-pathogenic oomycete Saprolegnia parasitica causes serious losses in aquaculture by infecting and killing freshwater fish. Like plant-pathogenic oomycetes, S. parasitica employs similar infection structures and secretes effector proteins that translocate into host cells to manipulate the host. Here, we show that the host-targeting protein SpHtp3 enters fish cells in a pathogen-independent manner. This uptake process is guided by a gp96-like receptor and can be inhibited by supramolecular tweezers. The C-terminus of SpHtp3 (containing the amino acid sequence YKARK), and not the N-terminal RxLR motif, is responsible for the uptake into host cells. Following translocation, SpHtp3 is released from vesicles into the cytoplasm by another host-targeting protein where it degrades nucleic acids. The effector translocation mechanism described here, is potentially also relevant for other pathogen–host interactions as gp96 is found in both animals and plants.

DATA

Data di pubblicazione: 14/06/2018

AUTORI

TRUSCH FRANZISKA
LOEBACH LARS
WAWRA STEPHAN
DURWARD ELAINE
WUENSCH ANDREAS
IBERAHIM NURUL AQILAH
DE BRUIJN IRENE
MACKENZIE KEVIN
WILLEMS ARIANE
TOLOCZKO ALEKSANDRA
DIEGUEZ-URIBEONDO JAVIER
RASMUSSEN TIM
SCHRADER THOMAS
BAYER PETER
SECOMBES CHRIS J
VAN WEST PIETER

RIVISTA

Nature Communications, 9, 2347

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