Pubblicazione scientifica

Genomic structure, expression, and functional characterization of checkpoint kinase 1 from Penaeus monodon

FILONE TECNOLOGICO	TEMA
produzione e raccolta

RIASSUNTO

Chk1 is a cell-cycle regulator. Chk1 has been identified in organisms ranging from yeast to humans, but few researchers have studied Chk1 in shrimps. We cloned Chk1 from the black tiger shrimp (Penaeus monodon). The full-length cDNA sequence of PmChk1 had 3,334 base pairs (bp), with an open reading frame of 1,455 bp. The complete genomic sequence of PmChk1 (11,081 bp) contained 10 exons separated by nine introns. qRT-PCR showed that PmChk1 was highly expressed in the ovaries and gills of P. monodon. The lowest PmChk1 expression was noted in stage III of ovarian development in P. monodon. PmChk1 expression decreased significantly after injection of 5-hydroxytryptamine and eyestalk ablation in P. monodon ovaries. RNA interference experiments were undertaken to examine the expression of PmChk1, PmCDC2, and PmCyclin B. PmChk1 knockdown in the ovaries and hepatopancreas by dsRNA-Chk1 was successful. The localization and level of PmChk1 expression in the hepatopancreas was studied using in situ hybridization, which showed that data were in accordance with those of qRT-PCR. The Gonadosomatic Index of P. monodon after dsRNA-Chk1 injection was significantly higher than that after injection of dsRNA-GFP or phosphate-buffered saline. These data suggest that PmChk1 may have important roles in the ovarian maturation of P. monodon.

DATA

Data di pubblicazione: 24/05/2018

AUTORI

QIU LIHUA
ZHAO CHAO
WANG PENGFEI
FAN SIGANG
YAN LULU
XIE BOBO
JIANG SHIGUI
WANG SHU
LIN HEIZHAO

ENTE DI AFFERENZA

SOUTH CHINA SEA FISHERIES RSCH INST CHINESE ACAD FISHERY SCIENCES KEY LAB
KEY LAB OF AQUATIC GENOMICS MINST AGRICULTURE BEIJING CHINA
SOUTH CHINA SEA RESOURCE EXPLOITATION & PROTECTION COLLABORATIVE INNOV CTR SUN YAT SEN UNIV GUANGZHOU CHINA
CHINESE ACAD FISHERY SCIENCES BEIJING CHINA
SHENZHEN BASE OF SOUTH CHINA SEA FISHERIES RSCH INST CHINESE ACAD FISHERY SCIENCES SHENZHEN PR CHINA
CHANG GUNG UNIV TAIWAN

RIVISTA

PLoS ONE, 13(5), e0198036

IMPACT FACTOR

2,870

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