Pubblicazione scientifica

Chemical Synthesis and Functional Analysis of VarvA Cyclotide

FILONE TECNOLOGICO	TEMA
produzione e raccolta
trasformazione e commercializzazione

RIASSUNTO

Cyclotides are circular peptides found in various plant families. A cyclized backbone, together with multiple disulfide bonds, confers the peptides’ exceptional stability against protease digestion and thermal denaturation. In addition, the features of these antimicrobial molecules make them suitable for use in animal farming, such as aquaculture. Fmoc solid phase peptide synthesis on 2-chlorotrityl chlorine (CTC) resin using the “tea-bag” approach was conducted to generate the VarvA cyclotide identified previously from Viola arvensis. MALDI-TOF mass spectrometry determined the correct peptide amino acid sequence and the cyclization sites-critical in this multicyclic compound. The cyclotide showed antimicrobial activity against various Gram-negative bacteria, including recurrent pathogens present in Chilean aquaculture. The highest antimicrobial activity was found to be against Flavobacterium psychrophilum. In addition, membrane blebbing on the bacterial surface after exposure to the cyclotide was visualized by SEM microscopy and the Sytox Green permeabilization assay showed the ability to disrupt the bacterial membrane. We postulate that this compound can be proposed for the control of fish farming infections.

DATA

Data di pubblicazione: 19/04/2018

AUTORI

ALVAREZ CLAUDIO A
SANTANA PAULA A
LUNA OMAR
CARDENAS CONSTANZA
ALBERICIO FERNANDO
ROMERO MARIA S
GUZMAN FANNY

ENTE DI AFFERENZA

LAB DE FISIOLOGIA Y GENETICA MARINA FIGEMA CTR ESTUDIOS AVANZADOS EN ZONAS ARIDAS CEAZA COQUIMBO 1781421 CHILE CLAUDIO ALVAREZ CEAZA CL
FACULTAD DE CIENCIAS DEL MAR UNIV CATOLICA DEL NORTE COQUIMBO 1781421 CHILE MSROMERO UCN CL
CTR AQUAPACIFICO COQUIMBO 1781421 CHILE
NUCLEO DE BIOTECNOLOGIA DE CURAUMA PONTIFICIA UNIV CATOLICA DE VALPARAISO VALPARAISO 2373223 CHILE PAULA SANTANA PUCV CL PAS OMAR LUNA G GMAIL COM OL CONSTANZA CARDENAS PUCV CL CC
DEPT ORGANIC CHEMISTRY & CIBER BBN NETWORKING CTR ON BIOENGINEERING BIOMATERIALS & NANOMEDICINE UNIV BARCELONA BARCELONA 08007 SPAIN ALBERICIO UKZN AC ZA
SCHOOL OF CHEMISTRY UNIV KWAZULU NATAL DURBAN 4001 SOUTH AFRICA

RIVISTA

Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(4), 952

IMPACT FACTOR

3,010

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