Pubblicazione scientifica

Coupling Plant-Derived Cyclotides to Metal Surfaces: An Antibacterial and Antibiofilm Study

FILONE TECNOLOGICO	TEMA
produzione e raccolta

RIASSUNTO

Modification of metal surfaces with antimicrobial peptides is a promising approach to reduce bacterial adhesion. Here, cyclic peptides or cycloids, possessing remarkable stability and antimicrobial activities, were extracted and purified from Viola philippica Cav., and identified using mass spectrometry. Cyclotides were subsequently utilized to modify stainless steel surfaces via polydopamine-mediated coupling. The resulting cyclotide-modified surfaces were characterized by Fourier transform infrared (FTIR) spectroscopy and contact angle analysis. The antibacterial capacity of these cyclotides against Staphylococcus aureus was assessed by Alamar blue assay. The antibiofilm capacity of the modified surfaces was assessed by crystal violet assay, and scanning electron microscopy (SEM). A composite of Kalata b1, Varv A, Viba 15 and Viba 17 (P1); Varv E (P2); and Viphi G (P3) were isolated and identified. FTIR analysis of the modified surfaces demonstrated that cyclotides bound to the surfaces and induced reduction of contact angles. Antimicrobial effects showed an order P3 > P1 and P2, with P3-treated surfaces demonstrating the strongest antibiofilm capacity. SEM confirmed reduced biofilm formation for P3-treated surfaces. This study provides novel evidence for cyclotides as a new class for development of antibacterial and antibiofilm agents.

DATA

Data di pubblicazione: 09/03/2018

AUTORI

CAO PAN
YANG YING
UCHE FIDELIA IJEOMA
HART SARAH RUTH
LI WEN-WU
YUAN CHENGQING

ENTE DI AFFERENZA

SCHOOL OF ENERGY & POWER ENG WUHAN UNIV TECH WUHAN 430063 CHINA CAOP WHUT EDU CN
INST SCIENCE & TECH IN MEDICINE KEELE UNIV STOKE ON TRENT ST4 7QB UK Y YANG KEELE AC UK YY FI UCHE KEELE AC UK FIU SR HART KEELE AC UK SRH

RIVISTA

International Journal of Molecular Sciences, 19(3), 793

IMPACT FACTOR

4,210

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