Pubblicazione scientifica

pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas)

FILONE TECNOLOGICO	TEMA
produzione e raccolta

RIASSUNTO

ABSTRACT

Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and salinity in initiating spermatozoa movement in the Pacific oyster, Crassostrea gigas. The acidic pH of the gonad (5.82±0.22) maintained sperm in the quiescent stage and initiation of flagellar movement was triggered by a sudden increase of spermatozoa external pH (pHe) when released in seawater (SW). At pH 6.4, percentage of motile spermatozoa was three times higher when they were activated in SW containing 30 mM NH4Cl, which alkalinizes internal pH (pHi) of spermatozoa, compared to NH4Cl-free SW, revealing the role of pHi in triggering sperm movement. Percentage of motile spermatozoa activated in Na+-free artificial seawater (ASW) was highly reduced compared to ASW, suggesting that change of pHi triggering sperm motility was mediated by a Na+/H+ exchanger. Motility and swimming speed were highest in salinities between 33.8 and 42.7‰ (within a range of 0 to 50 ‰), and pH values above 7.5 (within a range of 4.5 to 9.5).

DATA

Data di pubblicazione: 26/02/2018

AUTORI

BOULAIS MYRINA
SUQUET MARC
ARSENAULT-PERNET EVE JULIE
MALO FLORENT
QUEAU ISABELLE
PIGNET PATRICIA
RATISKOL DOMINIQUE
LE GRAND JACQUELINE
HUBER MATTHIAS
COSSON JACKY

ENTE DI AFFERENZA

CNRS UMR 6539 LEMAR UBO CNRS IRD IFREMER IUEM PLOUZANE 29280 FRANCE
IFREMER UMR 6539 LEMAR UBO CNRS IRD IFREMER SITE EXPERIMENTAL D ARGENTON LANDUNVEZ 29840 FRANCE
IFREMER LMEE CTR BRETAGNE PLOUZANE 29280 FRANCE
UNIV SOUTH BOHEMIA IN CESKE BUDEJOVICE FACULTY OF FISHERIES & PROTECTION OF WATERS SOUTH BOHEMIAN RSCH CTR AQUACULTURE & BIODIVERSITY OF HYDROCENOSES VODNANY 38925 CZECH REPUBLIC

RIVISTA

Biology Open, 7(3), bio031427

IMPACT FACTOR

1,980

indietro