Pubblicazione scientifica

Antibacterial Effects of Chitosan/Cationic Peptide Nanoparticles

FILONE TECNOLOGICO	TEMA
produzione e raccolta

RIASSUNTO

This study attempted to develop chitosan-based nanoparticles with increased stability and antibacterial activity. The chitosan/protamine hybrid nanoparticles were formed based on an ionic gelation method by mixing chitosan with protamine and subsequently cross-linking the mixtures with sodium tripolyphosphate (TPP). The effects of protamine on the chemical structures, physical properties, and antibacterial activities of the hybrid nanoparticles were investigated. The antibacterial experiments demonstrated that the addition of protamine (125 µg/mL) in the hybrid nanoparticles (500 µg/mL chitosan and 166.67 µg/mL TPP) improved the antimicrobial specificity with the minimum inhibitory concentration (MIC) value of 31.25 µg/mL towards Escherichia coli (E. coli), while the MIC value was higher than 250 µg/mL towards Bacillus cereus. The chitosan/protamine hybrid nanoparticles induced the formation of biofilm-like structure in B. cereus and non-motile-like structure in E. coli. The detection of bacterial cell ruptures showed that the inclusion of protamine in the hybrid nanoparticles caused different membrane permeability compared to chitosan nanoparticles and chitosan alone. The chitosan/protamine nanoparticles also exhibited lower binding affinity towards B. cereus than E. coli. The results suggested that the hybridization of chitosan with protamine improved the antibacterial activity of chitosan nanoparticles towards pathogenic E. coli, but the inhibitory effect against probiotic B. cereus was significantly reduced.

DATA

Data di pubblicazione: 05/02/2018

AUTORI

TAMARA FRANS RICARDO
LIN CHI
MI FWU-LONG
HO YI-CHENG

ENTE DI AFFERENZA

DEPT BIOAGRICULTURAL SCIENCE NATL CHIAYI UNIV CHIAYI 60004 TAIWAN ANSARDO GMAIL COM FRT MERVYNLIN11 GMAIL COM CL
GRADUATE INST MEDICAL SCIENCES COLLEGE OF MEDICINE TAIPEI MEDICAL UNIV TAIPEI 11031 TAIWAN
DEPT BIOCHEMISTRY & MOLECULAR CELL BIOLOGY SCHOOL OF MEDICINE TAIPEI MEDICAL UNIV TAIPEI 11031 TAIWAN
GRADUATE INST NANOMEDICINE & MEDICAL ENG COLLEGE OF BIOMEDICAL ENG TAIPEI MEDICAL UNIV TAIPEI 11031 TAIWAN

RIVISTA

Nanomaterials, 8(2), 88

IMPACT FACTOR

4,080

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