Pubblicazione scientifica

Comparative Proteomic Analysis of Lysine Acetylation in Fish CIK Cells Infected with Aquareovirus

FILONE TECNOLOGICO	TEMA
produzione e raccolta
trasformazione e commercializzazione

RIASSUNTO

Grass carp (Ctenopharyngodon idellus) is an important worldwide commercial freshwater culture species. However, grass carp reovirus (GCRV) causes serious hemorrhagic disease in fingerlings and yearlings of fishes. To understand the molecular pathogenesis of host cells during GCRV infection, intensive proteomic quantification analysis of lysine acetylation in Ctenopharyngodon idella kidney (CIK) cells was performed. Using dimethylation labeling-based quantitative proteomics, 832 acetylated proteins with 1391 lysine acetylation sites were identified in response to GCRV infection, among which 792 proteins with 1323 sites were quantifiable. Bioinformatics analysis showed that differentially expressed lysine acetylated proteins are involved in diverse cellular processes and associated with multifarious functions, suggesting that extensive intracellular activities were changed upon viral infection. In addition, extensive alterations on host–protein interactions at the lysine acetylation level were also detected. Further biological experiments showed that the histone deacetylases (HDAC) inhibitor suberoylanilide hydroxamic acid (SAHA) could significantly suppress the GCRV replication. To our knowledge, this is the first to reveal the proteome-wide changes in host cell acetylome with aquatic virus infection. The results provided in this study laid a basis for further understanding the host response to aquareovirus infection in the post-translational modification aspect by regulating cell lysine acetylation conducive to viral replication.

DATA

Data di pubblicazione: 14/11/2017

AUTORI

GUO HONG
ZHANG JIE
WANG YAPING
BU CHEN
ZHOU YANYAN
FANG QIN

ENTE DI AFFERENZA

STATE KEY LAB OF VIROLOGY WUHAN INST VIROLOGY CHINESE ACAD SCIENCES WUHAN 430071 CHINA GUOHONG WH IOV CN HG ZHANGJ WH IOV CN JZ
STATE KEY LAB OF FRESHWATER ECOLOGY & BIOTECHNOLOGY INST HYDROBIOLOGY CHINESE ACAD SCIENCES WUHAN 430072 CHINA WANGYP IHB AC CN
JINGJIE PTM BIOLAB HANGZHOU CO LTD

RIVISTA

International Journal of Molecular Sciences, 18(11), 2419

IMPACT FACTOR

4,210

indietro