Pubblicazione scientifica

Viral infection upregulates myostatin promoter activity in orange-spotted grouper (Epinephelus coioides)

FILONE TECNOLOGICO	TEMA
produzione e raccolta

RIASSUNTO

Myostatin is a negative regulator of myogenesis and has been suggested to be an important factor in the development of muscle wasting during viral infection. The objective of this study was to characterize the main regulatory element of the grouper myostatin promoter and to study changes in promoter activity due to viral stimulation. In vitro and in vivo experiments indicated that the E-box E6 is a positive cis-and trans-regulation motif, and an essential binding site for MyoD. In contrast, the E-box E5 is a dominant negative cis-regulatory. The characteristics of grouper myostatin promoter are similar in regulation of muscle growth to that of other species, but mainly through specific regulatory elements. According to these results, we conducted a study to investigate the effect of viral infection on myostatin promoter activity and its regulation. The nervous necrosis virus (NNV) treatment significantly induced myostatin promoter activity. The present study is the first report describing that specific myostatin motifs regulate promoter activity and response to viral infection.

DATA

Data di pubblicazione: 16/10/2017

AUTORI

CHEN YI-TIEN
LIN CHAO-FEN
CHEN YOUNG-MAO
LO CHIH-EN
CHEN WAN-ERH
CHEN TZONG-YUEH

ENTE DI AFFERENZA

LAB OF MOLECULAR GENETICS DEPT BIOTECHNOLOGY & BIOINDUSTRY SCIENCES COLLEGE OF BIOSCIENCE & BIOTECHNOLOGY NATL CHENG KUNG UNIV TAINAN TAIWAN
LAB OF MOLECULAR GENETICS INST BIOTECHNOLOGY COLLEGE OF BIOSCIENCE & BIOTECHNOLOGY NATL CHENG KUNG UNIV TAINAN TAIWAN
TRANSLATIONAL CTR MARINE BIOTECHNOLOGY NATL CHENG KUNG UNIV TAINAN TAIWAN
AGRICULTURE BIOTECHNOLOGY RSCH CTR NATL CHENG KUNG UNIV TAINAN TAIWAN
UNIV HONG KONG HONG KONG

RIVISTA

PLoS ONE, 12(10), e0186506

IMPACT FACTOR

2,870

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