Pubblicazione scientifica

Continuous selection pressure to improve temperature acclimation of Tisochrysis lutea

FILONE TECNOLOGICO	TEMA
produzione e raccolta

RIASSUNTO

Temperature plays a key role in outdoor industrial cultivation of microalgae. Improving the thermal tolerance of microalgae to both daily and seasonal temperature fluctuations can thus contribute to increase their annual productivity. A long term selection experiment was carried out to increase the thermal niche (temperature range for which the growth is possible) of a neutral lipid overproducing strain of Tisochrysis lutea. The experimental protocol consisted to submit cells to daily variations of temperature for 7 months. The stress intensity, defined as the amplitude of daily temperature variations, was progressively increased along successive selection cycles. Only the amplitude of the temperature variations were increased, the daily average temperature was kept constant along the experiment. This protocol resulted in a thermal niche increase by 3°C (+16.5%), with an enhancement by 9% of the maximal growth rate. The selection process also affected T. lutea physiology, with a feature generally observed for ‘cold-temperature’ type of adaptation. The amount of total and neutral lipids was significantly increased, and eventually productivity was increased by 34%. This seven month selection experiment, carried out in a highly dynamic environment, challenges some of the hypotheses classically advanced to explain the temperature response of microalgae.

DATA

Data di pubblicazione: 13/09/2017

AUTORI

BONNEFOND HUBERT
GRIMAUD GHJUVAN
RUMIN JUDITH
BOUGARAN GAEL
TALEC AMELIE
GACHELIN MANON
BOUTOUTE MARC
PRUVOST ERIC
BERNARD OLIVIER
SCIANDRA ANTOINE

ENTE DI AFFERENZA

SORBONNE UNIV UPMC UNIV PARIS 06 CNRS INSU LAB
INRIA BIOCORE SOPHIA ANTIPOLIS CEDEX FRANCE
IFREMER PBA NANTES FRANCE
INTL CTR GENETIC ENG & BIOTECHNOLOGY INDIA

RIVISTA

PLoS ONE, 12(9), e0183547

IMPACT FACTOR

2,870

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