Pubblicazione scientifica

Genetic dissection of endothelial transcriptional activity of zebrafish aryl hydrocarbon receptors (AHRs)

FILONE TECNOLOGICO	TEMA
produzione e raccolta
trasformazione e commercializzazione

RIASSUNTO

The aryl hydrocarbon receptor (AHR) is a basic helix-loop-helix transcription factor conserved across phyla from flies to humans. Activated by a number of endogenous ligands and environmental toxins, studies on AHR function and gene regulation have largely focused on a toxicological perspective relating to aromatic hydrocarbons generated by human activities and the often-deleterious effects of exposure on vertebrates mediated by AHR activation. A growing body of work has highlighted the importance of AHR in physiologic processes, including immune cell differentiation and vascular patterning. Here we dissect the contribution of the 3 zebrafish AHRs, ahr1a, ahr1b and ahr2, to endothelial cyp1a1/b1 gene regulation under physiologic conditions and upon exposure to the AHR ligand Beta-naphthoflavone. We show that in fish multiple AHRs are functional in the vasculature, with vessel-specific differences in the ability of ahr1b to compensate for the loss of ahr2 to maintain AHR signaling. We further provide evidence that AHR can regulate the expression of the chemokine receptor cxcr4a in endothelial cells, a regulatory mechanism that may provide insight into AHR function in the endothelium.

DATA

Data di pubblicazione: 17/08/2017

AUTORI

SUGDEN WADE W
LEONARDO-MENDONCA ROBERTO C
ACUNA-CASTROVIEJO DARIO
SIEKMANN ARNDT F

ENTE DI AFFERENZA

Cells-in-Motion Cluster of Excellence (EXC 1003 –CiM) University of Muenster Muenster Germany
MAX PLANCK INST MOLECULAR BIOMEDICINE MUENSTER GERMANY
UNIV ATLANTICA FABRICA DA POLVORA DE BARCARENA BARCARENA PORTUGAL
INST BIOTECNOLOGIA CTR INVESTIGACION BIOMEDICA PARQUE TECNOLOGICO DE CIENCIAS SALUD UNIV GRANADA GRANADA SPAIN
UNIDAD DE GESTION CLINICA DE LAB
TEXAS A & M UNIV US

RIVISTA

PLoS ONE, 12(8), e0183433

IMPACT FACTOR

2,870

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